pTRV2-MCS病毒介导的植物RNAi载体VIGS质粒图谱序列抗性说明书BioVector NTCC保藏中心
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- 货 号:pTRV2-MCS
- 产 地:北京
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Order ID | Name | Description |
BiovectorpTRV2-MCS | pTRV2-MCS | pTRV2-MCS,KanR,in E.coli. Storage:4℃ |
Recovery
1.Obtainan LB agar plate with the appropriate antibiotic.
2.Usinga sterile pipette tip, touch the bacteria growing within the punctured area ofthe stab culture. (A sterilized wire loop or sterile toothpick can be used inplace of a sterile pipette tip.)
3.Runthis tip lightly over a section of the plate, as shown in the figure, to createstreak #1.
4.Usinganother sterile pipette tip, pass through streak #1 and spread the bacteriaover a second section of the plate, to create streak #2.
5.Usinga third sterile pipette tip, pass through streak #2 and spread the bacteriaover the last section of the plate, to create streak #3.
6.Growovernight in a 37 o C incubator (unless a different growthtemperature is indicated on the plasmid datasheet).
7.Inthe morning, single colonies should be visible. If the bacterial growth is toodense, re-streak onto a new agar plate to obtain single colonies.
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