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pET-11d(+)图谱序列抗性价格Biovector质粒库

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pet11d载体基本信息

出品公司:别名:质粒类型:表达水平:克隆方法:载体大小:5' 测序引物:5' 测序引物序列:  载体标签:载体抗性:备注:产品目录号:稳定性:组成型:病毒/非病毒:
EMD Biosciences(Novagen)
pET11d, pet 11d
大肠杆菌蛋白表达
多克隆位点,限制性内切酶
5674bp
T7
5'-TAATACGACTCACTATAGGG-3'
N-T7
Ampicillin
Approximate size is 5675 bp. Hosts: E.coli. Related vectors: pBR322.
69439-3
瞬时表达 Transient
组成型 Constitutive
非病毒

pet11d载体质粒图谱和多克隆位点信息

pet11d载体图谱
pet11d多克隆位点

Feature Name
Start
End
 
T7_terminator316188
T7_Terminal_primer256274
T7_leader358326
T7_transl_en_RBS382366
lacO427400
T7_promoter445427
pBRrevBam_primer548529
lacI8231914
tet (576 - 1190)19732587
ROP32263417
pGEX_3_primer34333411
Ampicillin54664606
AmpR_promoter55365508
ORF
Start
End
 
ORF frame 35821082
ORF frame 19551914
ORF frame 123921697
ORF frame 219702587
ORF frame 254664606
Enzyme Name
Cut
ClaI24
HindIII29
BamHI319
NheI350
NcoI355
XbaI394
BglII460
BclI1196
ApaI1393
HpaI1688
EagI2250
NruI2285
MscI2757
PstI4924
AatII5601
EcoRI5672

pet11d载体简介

The pET-11a-d vectors carry an N-terminal T7•Tag® sequence and BamH I cloning site. Thesevectors are the precursors to many pET family vectors; the pET-21a-d(+) series corresponds topET-11a-d but incorporates several additional features. Unique sites are shown on the circle map.Note that the sequence is numbered by the pBR322 convention, so the T7 expression region isreversed on the circular map. The cloning/expression region of the coding strand transcribed byT7 RNA polymerase is shown below.

pet11d载体序列

LOCUS       pET11d    5674 bp     DNA  circular   SYN
DEFINITION  pET11d
ACCESSION   
KEYWORDS    
SOURCE      

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