pCDFDuet-1图谱序列抗性价格Biovector质粒库

价　　格：¥0
货　　号：39559699380
产　　地：北京

竭诚为您服务！

BioVector NTCC典型培养物保藏中心: 联系人：Dr.Xu, Biovector NTCC Inc.; 电话：400-800-2947 工作QQ:1843439339 (微信同号); 邮件：Biovector@163.com; 手机：18901268599; 地址：北京; 已注册

详细信息
咨询记录(共0条)

质粒载体菌株细胞株基因保藏中心-Biovector Science Lab,Inc.可提供数万种质粒载体、菌株、基因和细胞株,并可提供实验技术外包服务,如基因克隆、质粒构建、蛋白原核、真核表达及纯化、病毒包装、基因沉默RNAi等服务。作为美国质粒载体菌株细胞株基因保藏中心分库及唯一办事处，Biovector Inc.可为您提供便捷一站式的产品进口服务，到货快捷，步骤简便。通过Google搜索"Biovector+您所需资源名称",您一定能找到所需的资源和技术服务。如有需要请联系：

Dr Xu,Senior Scientist
电邮Biovector@163.com
电话1890I268599

订购合同下载请点击：Biovector质粒载体菌株细胞基因订购合同-Biovector Science Lab

^_^寻找您所需质粒载体菌株基因细胞株等资源的窍门：在Google，Baidu等搜索引擎中搜索“Biovector您所需的资源名称”结果即刻闪现，马上试试吧^_^

点击浏览器菜单“编辑”-->"查找"或者直接按“Ctrl+F”查找您所需资源

由于页面字数限制，尚有很多质粒载体菌株基因片段名称未列出，如查找不到您所需资源，请发邮件或电话查询。

Biovector,Inc质粒载体菌株细胞株基因保藏中心列表网址http://www.Biovector.net

标签：以下资源由Biovector Inc. 质粒载体菌株细胞株基因保藏中心独家供应并发布，侵权必究。Biovector,Inc质粒载体菌株细胞株基因保藏中心列表网址http://biovector.blog.163.com质粒载体索取报价价格优惠售价出售图谱序列酶切位点转化构建求购购买订购 sequence map plasmid vector gene strain order ship 抗性 resistance restriction site

产品分类

原核表达载体

pCDFDuet1载体基本信息

出品公司:别名:质粒类型:表达水平:克隆方法:载体大小:5' 测序引物:5' 测序引物序列: 3' 测序引物:3' 测序引物序列: 载体标签:载体抗性:备注:产品目录号:稳定性:组成型:病毒/非病毒:

EMD Biosciences (Novagen)

pCDFDuet1, pCDFDuet 1

大肠杆菌蛋白双表达载体，有两个多克隆位点

高

多克隆位点，限制性内切酶

3781 bp

T7: 5'-TAATACGACTCACTATAGGG-3';

T7t

T7t: 5'-TGCTAGTTATTGCTCAGCGG-3'

N-His, C-S

streptomycin

pCDFDuet-1 is designed for the coexpression of two target genes. pCDFDuet-1 carries the CloDF13 replicon, lacI gene and streptomycin/spectinomycin resistance gene (aadA).

71340-3

瞬时表达 Transient

组成型 Constitutive

非病毒

pCDFDuet1载体质粒图谱和多克隆位点信息

pCDFDuet-1载体图谱

pCDFDuet1载体简介

pCDFDue-1 is designed for the coexpression of two target ORFs. The vector contains two multiple cloning sites (MCS), each of which is preceded by a T7lac promoter and ribosome binding site (rbs). The vector also carries the CloDF13-derived CDF replicon, lacI gene and streptomycin/spectinomycin resistance gene (SmR). This vector can be used in combination with pACYCDuet™-1 (Cat. No. 71147-3), pRSFDuet™-1 (Cat. No. 71341-3), and pETDuet™-1 (Cat. No. 71146-3) in an appropriate host strain for the coexpression of 4 to 8 target proteins. ORFs inserted into MCS1 can be sequenced using the ACYCDuetUP1 Primer (Cat. No. 71178-3) and DuetDOWN1 Primer (Cat. No. 71179-3). ORFs inserted into MCS2 can be sequenced using the DuetUP2 Primer (Cat. No. 71180-3) and T7 Terminator Primer (Cat. No. 69337-3). Unique sites are shown on the circle map.

pCDFDuet1载体序列

LOCUS       pCDFDuet   3781 bp    DNA Circular    SYN
DEFINITION pCDFDuet
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTAUTHORNAME|biofeng.com|
COMMENT     VNTOAUTHORAD1|Novagen Inc.|
FEATURES             Location/Qualifiers
     source          1..3781
                     /organism="pCDFDuet"
                     /mol_type="other DNA"
     misc_feature    2..29
                     /label="lacO"
     promoter        214..232
                     /label="T7_promoter"
     misc_feature    232..259
                     /label="lacO"
     misc_feature    complement(448..466)
                     /label="T7_Terminal_primer"
     terminator      467..502
                     /label="T7_terminator"
     gene            complement(680..1471)
                     /label="spect (variant)"
                     /gene="spect (variant)"
     CDS             complement(680..1471)
                     /label="ORF frame 1"
     CDS             complement(680..1471)
                     /label="ORF frame 1"
     promoter        complement(1513..1541)
          &