pCDFDuet-1图谱序列抗性价格Biovector质粒库

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产品分类

原核表达载体

pCDFDuet1载体基本信息

出品公司:别名:质粒类型:表达水平:克隆方法:载体大小:5' 测序引物:5' 测序引物序列: 3' 测序引物:3' 测序引物序列: 载体标签:载体抗性:备注:产品目录号:稳定性:组成型:病毒/非病毒:

EMD Biosciences (Novagen)

pCDFDuet1, pCDFDuet 1

大肠杆菌蛋白双表达载体，有两个多克隆位点

高

多克隆位点，限制性内切酶

3781 bp

T7: 5'-TAATACGACTCACTATAGGG-3';

T7t

T7t: 5'-TGCTAGTTATTGCTCAGCGG-3'

N-His, C-S

streptomycin

pCDFDuet-1 is designed for the coexpression of two target genes. pCDFDuet-1 carries the CloDF13 replicon, lacI gene and streptomycin/spectinomycin resistance gene (aadA).

71340-3

瞬时表达 Transient

组成型 Constitutive

非病毒

pCDFDuet1载体质粒图谱和多克隆位点信息

pCDFDuet-1载体图谱

pCDFDuet1载体简介

pCDFDue-1 is designed for the coexpression of two target ORFs. The vector contains two multiple cloning sites (MCS), each of which is preceded by a T7lac promoter and ribosome binding site (rbs). The vector also carries the CloDF13-derived CDF replicon, lacI gene and streptomycin/spectinomycin resistance gene (SmR). This vector can be used in combination with pACYCDuet™-1 (Cat. No. 71147-3), pRSFDuet™-1 (Cat. No. 71341-3), and pETDuet™-1 (Cat. No. 71146-3) in an appropriate host strain for the coexpression of 4 to 8 target proteins. ORFs inserted into MCS1 can be sequenced using the ACYCDuetUP1 Primer (Cat. No. 71178-3) and DuetDOWN1 Primer (Cat. No. 71179-3). ORFs inserted into MCS2 can be sequenced using the DuetUP2 Primer (Cat. No. 71180-3) and T7 Terminator Primer (Cat. No. 69337-3). Unique sites are shown on the circle map.

pCDFDuet1载体序列

LOCUS       pCDFDuet   3781 bp    DNA Circular    SYN
DEFINITION pCDFDuet
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTAUTHORNAME|biofeng.com|
COMMENT     VNTOAUTHORAD1|Novagen Inc.|
FEATURES             Location/Qualifiers
     source          1..3781
                     /organism="pCDFDuet"
                     /mol_type="other DNA"
     misc_feature    2..29
                     /label="lacO"
     promoter        214..232
                     /label="T7_promoter"
     misc_feature    232..259
                     /label="lacO"
     misc_feature    complement(448..466)
                     /label="T7_Terminal_primer"
     terminator      467..502
                     /label="T7_terminator"
     gene            complement(680..1471)
                     /label="spect (variant)"
                     /gene="spect (variant)"
     CDS             complement(680..1471)
                     /label="ORF frame 1"
     CDS             complement(680..1471)
                     /label="ORF frame 1"
     promoter        complement(1513..1541)
          &