pCOLADuet-1图谱序列抗性价格Biovector质粒库

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pCOLADuet1载体基本信息

出品公司:别名:质粒类型:表达水平:克隆方法:载体大小:5' 测序引物:5' 测序引物序列: 3' 测序引物:3' 测序引物序列: 载体标签:载体抗性:备注:产品目录号:稳定性:组成型:病毒/非病毒:

EMD Biosciences (Novagen)

pCOLADuet1, pCOLADuet 1

大肠杆菌蛋白双表达载体，有两个多克隆位点

高

多克隆位点，限制性内切酶

3719 bp

T7: 5'-TAATACGACTCACTATAGGG-3';

T7t

T7t: 5'-TGCTAGTTATTGCTCAGCGG-3'

N-His, C-S

Kanamycin

pRSFDuet-1 carries the RSF1030 replicon, lacI gene and kanamycin resistance gene.

71406-3

瞬时表达 Transient

组成型 Constitutive

非病毒

pCOLADuet1载体质粒图谱和多克隆位点信息

pCOLADuet-1载体图谱

pCOLADuet1载体简介

pCOLADuet-1 is designed for the coexpression of two target genes from a single plasmid. The vector encodes two multiple cloning sites (MCS) each of which is preceded by a T7 promoter, lac operator, and ribosome binding site (rbs). MCS-1 encodes the six-amino acid His•Tag sequence for the creation of a N-terminal fusion and MCS2 encodes the 15 amino acid S•Tag™ peptide after the last restriction site for the creation of a C-terminal fusion if desired. Genes inserted into MCS-1 can be sequenced using the ACYCDuetUP1 Primer and DuetDOWN1 Primer. Genes inserted into MCS-2 can be sequenced using the DuetUP2 Primer and T7 Terminator Primer. The vector has the COLA replicon from ColA(1) and the kanamycin resistance gene. This vector can be transformed into the same cell with plasmids containing compatible origins of replication and drug resistance genes for coexpression of up to 8 target genes.

pCOLADuet1载体序列

LOCUS       pCOLADuet-1   3719 bp    DNA Circular SYN
DEFINITION pCOLADuet-1
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTAUTHORNAME|biofeng.com|
COMMENT     VNTOAUTHORAD1|Novagen Inc.|
FEATURES             Location/Qualifiers
     source          1..3719
                     /organism="pCOLADuet-1"
                     /mol_type="other DNA"
     misc_feature    2..29
                     /label="lacO"
     promoter        214..232
                     /label="T7_promoter"
     misc_feature    232..259
                     /label="lacO"
     misc_feature    complement(448..466)
                     /label="T7_Terminal_primer"
     gene            complement(739..1554)
                     /label="KanR2"
                     /gene="KanR2"
     CDS             complement(739..1554)
                     /label="ORF frame 3"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
                     GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
                     VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
                     RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"
     promoter        complement(1596..1624)
                     /label="AmpR_promoter"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
                     GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
                     VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
                     RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"
     misc_feature    complement(2497..3588)
                     /label="lacI"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
                     GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
                     VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
                     RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"
     CDS             complement(2497..3456)
                     /label="ORF frame 3"
                     /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
                     RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
                     PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
                     HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
                     SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
                     LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
     CDS             complement(3329..158)
                     /label="ORF frame 1"
                     /translation="MRPQACRPAGAPSSNSDPGCGDDGDGCCPWYISLLKLNKIISTG
                     ELLSAHNSPIVSRINFLMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGECET
                     SNVIRCRRVCRCLLSDRFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQTV
                     VADWRCHLQSGPARAVANCRGD*"
     promoter        3703..2
                     /label="T7_promoter"
                     /translation="MRPQACRPAGAPSSNSDPGCGDDGDGCCPWYISLLKLNKIISTG
                     ELLSAHNSPIVSRINFLMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGECET
                     SNVIRCRRVCRCLLSDRFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQTV
                     VADWRCHLQSGPARAVANCRGD*"
ORIGIN
    1 ggggaattgt gagcggataa caattcccct gtagaaataa ttttgtttaa ctttaataag
   61 gagatatacc atgggcagca gccatcacca tcatcaccac agccaggatc cgaattcgag
121 ctcggcgcgc ctgcaggtcg acaagcttgc ggccgcataa tgcttaagtc gaacagaaag
181 taatcgtatt gtacacggcc gcataatcga aattaatacg actcactata ggggaattgt
241 gagcggataa caattcccca tcttagtata ttagttaagt ataagaagga gatatacata
301 tggcagatct caattggata tcggccggcc acgcgatcgc tgacgtcggt accctcgagt
361 ctggtaaaga aaccgctgct gcgaaatttg aacgccagca catggactcg tctactagcg
421 cagcttaatt aacctaggct gctgccaccg ctgagcaata actagcataa ccccttgggg
481 cctctaaacg ggtcttgagg ggttttttgc tgaaacctca ggcatttgag aagcacacgg
541 tcacactgct tccggtagtc aataaaccgg taaaccagca atagacataa gcggctattt
601 aacgaccctg ccctgaaccg acgacaagct gacgaccggg tctccgcaag tggcactttt
661 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
721 ccgctcatga attaattctt agaaaaactc atcgagcatc aaatgaaact gcaatttatt
781 catatcagga ttatcaatac catatttttg aaaaagccgt ttctgtaatg aaggagaaaa
841 ctcaccgagg cagttccata ggatggcaag atcctggtat cggtctgcga ttccgactcg
901 tccaacatca atacaaccta ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa
961 atcaccatga gtgacgactg aatccggtga gaatggcaaa agtttatgca tttctttcca
1021 gacttgttca acaggccagc cattacgctc gtcatcaaaa tcactcgcat caaccaaacc
1081 gttattcatt cgtgattgcg cctgagcgag acgaaatacg cggtcgctgt taaaaggaca
1141 attacaaaca ggaatcgaat gcaaccggcg caggaacact gccagcgcat caacaatatt
1201 ttcacctgaa tcaggatatt cttctaatac ctggaatgct gttttcccgg ggatcgcagt
1261 ggtgagtaac catgcatcat caggagtacg gataaaatgc ttgatggtcg gaagaggcat
1321 aaattccgtc agccagttta gtctgaccat ctcatctgta acatcattgg caacgctacc
1381 tttgccatgt ttcagaaaca actctggcgc atcgggcttc ccatacaatc gatagattgt
1441 cgcacctgat tgcccgacat tatcgcgagc ccatttatac ccatataaat cagcatccat
1501 gttggaattt aatcgcggcc tagagcaaga cgtttcccgt tgaatatggc tcatactctt
1561 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
1621 tgaatgtatt tagaaaaata aacaaatagg catgctagcg cagaaacgtc ctagaagatg
1681 ccaggaggat acttagcaga gagacaataa ggccggagcg aagccgtttt tccataggct
1741 ccgcccccct gacgaacatc acgaaatctg acgctcaaat cagtggtggc gaaacccgac
1801 aggactataa agataccagg cgtttccccc tgatggctcc ctcttgcgct ctcctgttcc
1861 cgtcctgcgg cgtccgtgtt gtggtggagg ctttacccaa atcaccacgt cccgttccgt
1921 gtagacagtt cgctccaagc tgggctgtgt gcaagaaccc cccgttcagc ccgactgctg
1981 cgccttatcc ggtaactatc atcttgagtc caacccggaa agacacgaca aaacgccact
2041 ggcagcagcc attggtaact gagaattagt ggatttagat atcgagagtc ttgaagtggt
2101 ggcctaacag aggctacact gaaaggacag tattt