pET-15b图谱序列抗性价格Biovector质粒库

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pet15b载体基本信息

出品公司:别名:质粒类型:表达水平:克隆方法:载体大小:5' 测序引物:5' 测序引物序列: 载体标签:载体抗性:备注:产品目录号:稳定性:组成型:病毒/非病毒:

EMD Biosciences(Novagen)

pET15b, pet 15b

大肠杆菌蛋白表达

高

多克隆位点，限制性内切酶

5708bp

5'-TAATACGACTCACTATAGGG-3'

N-His, N-thrombin

Ampicillin

Hosts: E.coli. Related vectors: pBR322.

69661-3

瞬时表达 Transient

组成型 Constitutive

非病毒

pet15b载体质粒图谱和多克隆位点信息

pet15b载体图谱

Feature Name
Start
End

T7_terminator	316	188
T7_Terminal_primer	256	274
T7_transl_en_RBS	416	400
lacO	461	434
T7_promoter	479	461
pBRrevBam_primer	582	563
lacI	857	1948
tet (576 - 1190)	2007	2621
ROP	3260	3451
pGEX_3_primer	3467	3445
Ampicillin	5500	4640
AmpR_promoter	5570	5542

ORF
Start
End

ORF frame 1	616	1116
ORF frame 2	989	1948
ORF frame 1	2426	1731
ORF frame 3	2004	2621
ORF frame 2	5500	4640

Enzyme Name
Cut

ClaI	24
HindIII	29
BamHI	319
XhoI	324
NdeI	331
NcoI	389
XbaI	428
BglII	494
BclI	1230
ApaI	1427
HpaI	1722
EagI	2284
NruI	2319
MscI	2791
PstI	4958
AatII	5635
EcoRI	5706

pet15b载体简介

The pET-15b vector (Cat. No. 69661-3) carries an N-terminal His•Tag® sequence followed by a thrombin site and three cloning sites. Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circular map. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase is shown below.

pet15b载体序列

1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
181 gcgggatatc cggatatagt tcctcctttc agcaaaaaac ccctcaagac ccgtttagag
241 gccccaaggg gttatgctag ttattgctca gcggtggcag cagccaactc agcttccttt
301 cgggctttgt tagcagccgg atcctcgagc atatggctgc cgcgcggcac caggccgctg
361 ctgtgatgat gatgatgatg gctgctgccc atggtatatc tccttcttaa agttaaacaa
421 aattatttct agaggggaat tgttatccgc tcacaattcc cctatagtga gtcgtattaa
481 tttcgcggga tcgagatctc gatcctctac gccggacgca tcgtggccgg catcaccggc
541 gccacaggtg cggttgctgg cgcctatatc gccgacatca ccgatgggga agatcgggct
601 cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc
661 gggggactgt tgggcgccat ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac
721 ggcctcaacc tactactggg ctgcttccta atgcaggagt cgcataaggg agagcgtcga
781 gatcccggac accatcgaat ggcgcaaaac ctttcgcggt atggcatgat agcgcccgga
841 agagagtcaa ttcagggtgg tgaatgtgaa accagtaacg ttatacgatg tcgcagagta
901 tgccggtgtc tcttatcaga ccgtttcccg cgtggtgaac caggccagcc acgtttctgc
961 gaaaacgcgg gaaaaagtgg aagcggcgat ggcggagctg aattacattc ccaaccgcgt
1021 ggcacaacaa ctggcgggca aacagtcgtt gctgattggc gttgccacct ccagtctggc
1081 cctgcacgcg ccgtcgcaaa ttgtcgcggc gattaaatct cgcgccgatc aactgggtgc
1141 cagcgtggtg gtgtcgatgg tagaacgaag cggcgtcgaa gcctgtaaag cggcggtgca
1201 caatcttctc gcgcaacgcg tcagtgggct gatcattaac tatccgctgg atgaccagga
1261 tgccattgct gtggaagctg cctgcactaa tgttccggcg ttatttcttg atgtctctga
1321 ccagacaccc atcaacagta ttattttctc ccatgaagac ggtacgcgac tgggcgtgga
1381 gcatctggtc gcattgggtc accagcaaat cgcgctgtta gcgggcccat taagttctgt
1441 ctcggcgcgt ctgcgtctgg ctggctggca taaatatctc actcgcaatc aaattcagcc
1501 gatagcggaa cgggaaggcg actggagtgc catgtccggt tttcaacaaa ccatgcaaat
1561 gctgaatgag ggcatcgttc ccactgcgat gctggttgcc aacgatcaga tggcgctggg
1621 cgcaatgcgc gccattaccg agtccgggct gcgcgttggt gcggatatct cggtagtggg
1681 atacgacgat accgaagaca gctcatgtta tatcccgccg ttaaccacca tcaaacagga
1741 ttttcgcctg ctggggcaaa ccagcgtgga ccgcttgctg caactctctc agggccaggc
1801 ggtgaagggc aatcagctgt tgcccgtctc actggtgaaa agaaaaacca ccctggcgcc
1861 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
1921 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtaagt tagctcactc
1981 attaggcacc gggatctcga ccgatgccct tgagagcctt caacccagtc agctccttcc
2041 ggtgggcgcg gggcatgact atcgtcgccg cacttatgac tgtcttcttt atcatgcaac
2101 tcgtaggaca ggtgccggca gcgctctggg tcattttcgg cgaggaccgc tttcgctgga
2161 gcgcgacgat gatcggcctg tcgcttgcgg tattcggaat cttgcacgcc ctcgctcaag
2221 ccttcgtcac tggtcccgcc accaaacgtt tcggcgagaa gcaggccatt atcgccggca
2281 tggcggccga cgcgctgggc tacgtcttgc tggcgttcgc gacgcgaggc tggatggcct
2341 tccccattat gattcttctc gcttccggcg gcatcgggat gcccgcgttg caggccatgc
2401 tgtccaggca ggtagatgac gaccatcagg gacagcttca aggatcgctc gcg