BPH-1 cell line人前列腺增生细胞-BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心
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- 货 号：BPH-1人前列腺增生细胞
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BPH-1人前列腺增生细胞
BPH-1 cell line
Cat No.: NTCC595981
Cell line:
BPH-1
Species:
human (Homo sapiens)
Cell type:
benign prostate hyperplasia
Origin:
prostate epithelial cells from a 68-year-old man with benign prostate hyperplasia; cells were immortalized
with SV-40 large T-antigen; cells were described in the literature to express cytokeratins 8, 18, and 19 (but
not 14) and to metabolize prostatic androgens
Biosafety level:
1
Permissions and restrictions:
A
Cell Culture Data:
Morphology:
adherent epitheloid cells growing as monolayers;
image
Medium:
80% RPMI 1640 + 20% h.i. FBS + 20 ng/ml testosterone + 5 µg/ml transferrin + 5 ng/ml sodium selenite + 5
µg/ml insulin
Subculture:
split confluent culture 1:3 to 1:8 every 3-7 days using trypsin/EDTA (cells should be incubated for more than
10 min with trypsin/EDTA at 37 °C); seed out at 0.1 x 105cells/cm2
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 50 hours
Harvest:
cell harvest of ca. 0.4 x 105cells/cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 +, cytokeratin-18 +, cytokeratin-19 +, desmin -,
endothel -, EpCAM +, GFAP -, neurofilament -, vimentin (+)
Fingerprint:
multiplex PCR of minisatellite markers revealed a unique DNA profile
Species:
confirmed as human with IEF of AST, LDH, NP
Cytogenetics:
human hypertriploid karyotype with 2.5% polyploidy - 75(69-79)<3n>XXY, -4, +5, +6, +7, -10, +11, -12, +15,
+21, +3mar - +dmin - i(3q), add(7)(p11), i(8q), add(10)(p13) - sideline with add(13)(p12) and smaller 10p+
marker - matches published karyotype - carries large submetacentric 10p+ marker
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -,
MLV -, SMRV -
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