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F-36P骨髓增生异常综合征细胞(MDS)
BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心
| Comment | GM-CSF- or IL-3-dependent cell line. Differentiate to erythoid cells with erythropoietin. | |||
| Animal | _human < Mammals | ||
| Genus | Homo | ||
| Species | sapiens | ||
| Race | Japanese | ||
| Gender | Male | ||
| Age at sampling | 65 years | ||
| Tissue | bone marrow | ||
| Disease name | myelodysplastic syndrome | ||
| Classification | cancer | ||
| Year of origin | 1989 | ||
| Lifespan | infinite | ||
| Morphology | lymphocyte-like | ||
| Medium | |||
| Culture type | Suspension cells | ||
| Culture medium | RPMI1640 + 5% FBS + 5ng/ml human IL-3 | ||
| Culture method | |||
| Antibiotics | Free | ||
| Passage method | dilution | ||
| Culture information | Passage ratio | 1 : 2-8 split | |
| SC frequency | Subculture : 2 times/week | ||
| Temperature | 37 ℃ | ||
| CO2 concentration | 5 % | ||
| Freeze medium | Medium + 10% DMSO | ||
| Freezing method | Slow freezing | ||
| Mycoplasma | (-) | ||
| Virus (HIV) | (-) | ||
| Virus (HTLV-1) | (-) | ||
| Isozyme | LD, NP | ||
| Chromosome mode | 39-122(50) : /40(1),41(4),42(8),43(17),44(17),85(1)/ | ||
| STR(human) | OK | ||
BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心
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