OCI-AML2人急性髓系白血病细胞-BioVector NTCC保藏中心

价　　格：¥14920
货　　号：OCI-AML2人急性髓系白血病细胞
产　　地：北京

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Cell line:: OCI-AML2
DSMZ no.:: ACC 99
Species:: human (Homo sapiens)
Cell type:: acute myeloid leukemia
Origin:: established from the peripheral blood of a 65-year-old man with acute myeloid leukemia (AML FAB M4) in 1986 at diagnosis; cells were described to express the retinoic acid receptor gene; cell line carries the DNMT3A R635W mutation
Reference(s):: 15050, 16667
Biosafety level:: 1
Permissions and restrictions:: <a href="https://www.dsmz.de/?id=295" "="" class="internal-link-new-window" style="color: rgb(22, 22, 22); text-decoration: none; border-bottom-width: 1px; border-bottom-style: dotted; border-bottom-color: rgb(124, 124, 124); background-image: url(https://www.dsmz.de/fileadmin/templates/gfx/link-extern.gif); padding-right: 12px; background-position: 100% 50%; background-repeat: no-repeat no-repeat;">A
: DSMZ Cell Culture Data:
Morphology:: single, round to oval cells in suspension
Medium:: 80-90% alpha-MEM + 10-20% h.i. FBS
Subculture:: maintain at 0.5-1.5 x 10⁶ cells/ml; seed out at ca. 0.5 x 10⁶ cells/ml; possibly no cell growth during first 3-4 days; upon thawing viability drops to about 50%
Incubation:: at 37 °C with 5% CO₂
Doubling time:: about 30-50 hours
Harvest:: maximal density at about 4 x 10⁶ cells/ml
Storage:: frozen with 70% medium, 20% FBS, 10% DMSO
: DSMZ Scientific Data:
Mycoplasma:: negative in DAPI, microbiological culture, RNA hybridization assays
Immunology:: CD3 -, CD4 +, CD13 +, CD14 -, CD15 +, CD19 -, CD33 +, CD34 -, CD68 +, HLA-DR (+); image
Fingerprint:: multiplex PCR of minisatellite markers revealed a unique DNA profile
Species:: confirmed as human with IEF of MDH, NP
Cytogenetics:: human hyperdiploid karyotype with 3.8% polyploidy - 48(43-49)<2n>XY, +6, +8, der(1)inv(1)(p36q31)t(1;6) (q13;p12), der(2)t(2;17)(p23;q24.1)del(2)(q14.2q36), der(3)t(1;3)(p36;p25), ins(3;2)(q21;q14.2q36), t(5;8)(q11.2;q24), der(6)t(1;6)(q31;p12)t(3;6) (q26;q24), inv(12)(p13.3q13.2), t(13;14)(q32/33;q24.2), der(17)t(2;17)(p23;q24.1) - sideline with +der(5) - carries apparent variant translocations involving several ANLL breakpoints: 1p36, 3q21, 3q26 (megakaryocytic abnormalities), 12p13, 17q24 (FAB-M4)
Viruses:: ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV -, HTLV-I/II -, MLV -, SMRV -