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MSCV-IRES-Thy1.1 DEST 逆病毒载体质粒图谱序列抗性

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MSCV-IRES-Thy1.1 DEST 
(BioVector-017442)

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Available to Academic and Nonprofits Only

BACKBONE

  • Vector backbone
    MSCV-IRES-Thy1.1
  • Backbone manufacturer
    Philippa Marrack Lab
  • Backbone size (bp)6063
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Thy1.1

GROWTH IN BACTERIA

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

SEQUENCE INFORMATION

  • Depositor Sequences
    None.
  • Addgene Sequences

Full plasmid sequence is available only if provided by the depositing laboratory.

GENE/INSERT

  • Gene/Insert name
    None

CLONING INFORMATION

  • Cloning methodRestriction Enzyme
  • 5′ cloning siteattB1 (not destroyed)
  • 3′ cloning siteattB2 (not destroyed)
  • 5′ sequencing primerpBABE5
  • 3′ sequencing primerIRES-R
  • (Common Sequencing Primers)

RESOURCE INFORMATION

DEPOSITOR COMMENTS

Recombination sites were added to MSCV-IRES-Thy1.1 for subcloning from pENTR vectors.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MSCV-IRES-Thy1.1 DEST was a gift from Anjana Rao (Addgene plasmid # 17442)
  • For your References section:

    FOXP3 controls regulatory T cell function through cooperation with NFAT. Wu Y, Borde M, Heissmeyer V, Feuerer M, Lapan AD, Stroud JC, Bates DL, Guo L, Han A, Ziegler SF, Mathis D, Benoist C, Chen L, Rao A. Cell. 2006 Jul 28. 126(2):375-87. 10.1016/j.cell.2006.05.042 PubMed 16873067

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