BACKBONE

• Vector backbone
  pBABE-puro
  (Search Vector Database)
• Backbone manufacturer
  Weinberg lab (Addgene#:1764)
• Backbone size (bp)5169
• Modifications to backbone
  Non-targeting complementary sites (7x in tandem) to serve as a negative control for microRNA sponge-mediated inhibition of specific microRNA function; suitable for stable expression. Note that EGFP is also cloned into this backbone, upstream of the sponge.
• Vector type
  Mammalian Expression, Retroviral
• Selectable markers
  Puromycin

GROWTH IN BACTERIA

• Bacterial Resistance(s)
  Ampicillin
• Growth Temperature
  37°C
• Growth Strain(s)
  DH5alpha
• Copy number
  High Copy

SEQUENCE INFORMATION

• Depositor Sequences
  None.
• Addgene Sequences
  Partial (2)

Full plasmid sequence is available only if provided by the depositing laboratory.

GENE/INSERT

• Gene/Insert name
  CXCR4 control sponge
• Alt name
  stable control sponge
• Insert Size (bp)
  2000

CLONING INFORMATION

• Cloning methodRestriction Enzyme
• 5′ cloning siteBamHI (unknown if destroyed)
• 3′ cloning siteApaI (unknown if destroyed)
• 5′ sequencing primerpBabe-5
• 3′ sequencing primerpBabe-3
(Common Sequencing Primers)

RESOURCE INFORMATION

• Terms and Licenses
  • UBMTA