首页 » pCLE逆病毒载体质粒图谱序列抗性

pCLE逆病毒载体质粒图谱序列抗性

  • 价  格:¥0
  • 货  号:3558713
  • 产  地:北京
点击询问我要采购
 竭诚为您服务!
BioVector NTCC典型培养物保藏中心
联系人:Dr.Xu, Biovector NTCC Inc.

电话:400-800-2947 工作QQ:1843439339 (微信同号)

邮件:Biovector@163.com

手机:18901268599

地址:北京

已注册
 

 

  • pCLE 
    (BioVector-017703)

    Add to Cart
    Available to Academic and Nonprofits Only

    BACKBONE

    GROWTH IN BACTERIA

    • Bacterial Resistance(s)
      Ampicillin
    • Growth Temperature
      37°C
    • Growth Strain(s)
      DH5alpha
    • Copy number
      High Copy

    SEQUENCE INFORMATION

    • Depositor Sequences
      None.
    • Addgene Sequences

    Full plasmid sequence is available only if provided by the depositing laboratory.

    GENE/INSERT

    • Gene/Insert name
      None

    CLONING INFORMATION

    • Cloning methodRestriction Enzyme
    • 5′ cloning siteBglII (not destroyed)
    • 3′ cloning siteBglII (not destroyed)
    • 5′ sequencing primerna
    • (Common Sequencing Primers)

    RESOURCE INFORMATION

    • Terms and Licenses

    DEPOSITOR COMMENTS

    pCLE includes a modified 5 LTR (CMV/MLV) to obtain higher titers, as well as the Xenopus EF1alpha enhancer/promoter to improve vector expression in the brain. In addition, the vector is dicistronic and utilizes an IRES to allow translation of the reporter gene, human placental alkaline phosphatase (PLAP).

    How to cite this plasmid

    These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

    • For your Materials & Methods section:

      pCLE was a gift from Nicholas Gaiano (Addgene plasmid # 17703)
    • For your References section:

      Radial glial identity is promoted by Notch1 signaling in the murine forebrain. Gaiano N, Nye JS, Fishell G. Neuron. 2000 May . 26(2):395-404. 10.1016/S0896-6273(00)81172-1 PubMed 10839358

 

您正在向 biovector.net  发送关于产品 pCLE逆病毒载体质粒图谱序列抗性 的询问

点击“立即发送”后,我们将在1个工作日内与您取得联系。