首页 » pMSCV-IRES-GFP II (pMIG II) 逆病毒载体质粒图谱序列抗性-BioVector NTCC保藏中心提供

pMSCV-IRES-GFP II (pMIG II) 逆病毒载体质粒图谱序列抗性-BioVector NTCC保藏中心提供

  • 价  格:¥2930
  • 货  号:pMSCV-IRES-GFP II pMIG II
  • 产  地:北京
点击询问我要采购
 竭诚为您服务!
BioVector NTCC典型培养物保藏中心
联系人:Dr.Xu, Biovector NTCC Inc.

电话:400-800-2947 工作QQ:1843439339 (微信同号)

邮件:Biovector@163.com

手机:18901268599

地址:北京

已注册
 


pMSCV-IRES-GFP II (pMIG II)
(BioVector-052107)


  • Enlarge

  • Download

  • PURPOSE
    (Empty Backbone) a bicistronic IRES-GFP containing retroviral vector, MCS is expanded from pMSCV-IRES-GFP
  • DEPOSITING LAB
    Dario Vignali
  • PUBLICATION
    Holst et al Nat Protoc. 2006;1(1):406-17.



BACKBONE

  • Vector backbone
    MSCV IRES GFP
    (Search Vector Database)
  • Modifications to backbone
    expanded MCS
  • Vector type
    Mammalian Expression, Retroviral
  • Tag / Fusion Protein

    • IRES-EGFP (C terminal on backbone)


GROWTH IN BACTERIA

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

SEQUENCE INFORMATION

  • Depositor Sequences
    Partial (1)
  • Addgene Sequences
    Partial (2)

Full plasmid sequence is available only if provided by the depositing laboratory.

CLONING INFORMATION

  • Cloning methodRestriction Enzyme

  • 5′ sequencing primerpLXSN_5_primer

(Common Sequencing Primers)

RESOURCE INFORMATION

  • Terms and Licenses
    • UBMTA

    • Ancillary Agreement for Plasmids Containing FP Materials

DEPOSITOR COMMENTS

The FP can be removed with Nco1-Not1 digest.
The Multicloning site reads EcoR1-BglII-SnaB1-BamH1-Mfe1-Xho1. These are unique. The Nhe1 site is not unique.

How to cite this plasmid






  • For your Materials & Methods section:

    pMSCV-IRES-GFP II (pMIG II) was a gift from Dario Vignali (Addgene plasmid # 52107)
  • For your References section:

    Generation of T-cell receptor retrogenic mice. Holst J, Szymczak-Workman AL, Vignali KM, Burton AR, Workman CJ, Vignali DA. Nat Protoc. 2006;1(1):406-17. 10.1038/nprot.2006.61 PubMed 17406263


您正在向 biovector.net  发送关于产品 pMSCV-IRES-GFP II (pMIG II) 逆病毒载体质粒图谱序列抗性-BioVector NTCC保藏中心提供 的询问

点击“立即发送”后,我们将在1个工作日内与您取得联系。