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MIEG-hCD4
(BioVector-040569)-
PURPOSE(Empty Backbone)
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DEPOSITING LABS
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PUBLICATION
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PRICE$65 (USD)
Add to CartAvailable to Academic and Nonprofits OnlyBACKBONE
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Vector backbonepMIEG3
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Backbone manufacturerDavid A. Williams (PMID: 10961859)
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Modifications to backboneIRES EGFP of pMIEG replaced with human CD4 cDNA
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Vector typeMammalian Expression, Retroviral
- Tag / Fusion Protein
- IRES hCD4 (C terminal on backbone)
GROWTH IN BACTERIA
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Bacterial Resistance(s)Ampicillin
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberHigh Copy
SEQUENCE INFORMATION
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Depositor SequencesNone.
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Addgene Sequences
Full plasmid sequence is available only if provided by the depositing laboratory.
CLONING INFORMATION
- Cloning methodRestriction Enzyme
- 5′ sequencing primerpLXSN_5
- 3′ sequencing primerIRES-R2 (gacggcaatatggtggaaa) (Common Sequencing Primers)
RESOURCE INFORMATION
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A portion of this plasmid was derived from a plasmid made byCloned human CD4 cDNA provided by G. Alkhatib, Indiana University.
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Terms and Licenses
DEPOSITOR COMMENTS
Retroviral vector MIEG-hCD4 was made by replacing the EGFP of the MIEG-EGFP vector with the hCD4 cDNA amplified from a cloned cDNA (provided by G. Alkhatib, Indiana University).
How to cite this plasmidThese plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MIEG-hCD4 was a gift from Alexander Dent & Mark Kaplan (Addgene plasmid # 40569) -
For your References section:
PU.1 expression delineates heterogeneity in primary Th2 cells. Chang HC, Zhang S, Thieu VT, Slee RB, Bruns HA, Laribee RN, Klemsz MJ, Kaplan MH. Immunity. 2005 Jun;22(6):693-703. 10.1016/j.immuni.2005.03.016 PubMed 15963784
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