首页 » pC-YFP植物双分子荧光互补载体BiFC质粒-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心

pC-YFP植物双分子荧光互补载体BiFC质粒-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心

  • 价  格:¥19532
  • 货  号:pC-YFP植物双分子荧光互补载体BiFC质粒
  • 产  地:北京
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pC-YFP植物双分子荧光互补载体BiFC质粒

BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心www.biovector.net


BiFC System info:


This Bimolecular Fluorescence Complementation system is based on the 172

N-terminal amino acids of EYFP, and the 85 C-terminal aa.s of ECFP (aa.s 154-239).

Use of the 4 different vectors yields C- and N-terminal fusions of a putative interactor

protein to both of the of the fluorescent protein halves.

All 4 vectors are binary with a backbone derived from pPZP312, a single 35S promoter

and a CaMV terminator (derived from pRT100).

The vectors confer Spectinomycin resistance in E. coli and Agrobacterium, and Basta

resistance in plants.

All vectors contain a 6xHis-tag allowing for simple affinity-based purification of the

expressed proteins.


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