首页 » AB.9 Cell Line斑马鱼尾鳍细胞株-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心

AB.9 Cell Line斑马鱼尾鳍细胞株-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心

  • 价  格:¥19300
  • 货  号:AB.9 Cell Line
  • 产  地:北京
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AB.9 Cell Line

Cat No.: NTCC5194101




Organism



Danio
   rerio
, zebrafish



Tissue



caudal fin



Cell Type



fibroblast



Product Format



frozen



Morphology



fibroblast



Biosafety
   Level



1



Age



adult



Strain



AB



Applications



This strain of zebrafish is used for genetic mapping.
   It was used used by Leonard I. Zon at Harvard to generate the
   zebrafish YAC genomic library and by Peter Goodfellow at Cambridge, United
   Kingdom to generate the zebrafish radiation hybrid panel.



Derivation



AB.9 is a fibroblast cell line derived from amputated
   caudal fins of an adult zebrafish, strain AB.



Complete
   Growth Medium



The base medium for this cell line is NTCC-formulated
   Dulbecco's Modified Eagle's medium, Catalog No. 30-2002. To make the complete
   growth medium, add the following components to the base medium:
   heat-inactivated fetal bovine serum to a final concentration of 15%.



Subculturing



Volumes used in this protocol are for 75 cm2 flask;
   proportionally reduce or increase amount of dissociation medium for culture
   vessels of other sizes.


1.
Remove and discard culture medium.


2.
Briefly rinse the cell layer with 0.25%
   (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which
   contains trypsin inhibitor.


3.
Add 2.0 to 3.0 mL of Trypsin-EDTA
   solution to flask and observe cells under an inverted microscope until cell
   layer is dispersed (usually within 5 to 15 minutes).

   Note: To avoid clumping do not agitate the cells by hitting or shaking
   the flask while waiting for the cells to detach. Cells that are difficult to
   detach may be placed at 28°C to facilitate dispersal.


4.
Add 6.0 to 8.0 mL of complete growth
   medium and aspirate cells by gently pipetting.


5.
Add appropriate aliquots of the cell
   suspension to new culture vessels.


6.
Incubate cultures at 28°C.


Subcultivation Ratio: A subcultivation ratio of
   1:3 to 1:4 is recommended


Medium Renewal: Every 2 to 3 days


Note: For more information on enzymatic
   dissociation and subculturing of cell lines consult Chapter 10 in Culture
   of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd
   edition, published by Alan R. Liss, N.Y., 1994.



Cryopreservation



Complete growth medium, 95%; DMSO, 5%



Culture
   Conditions



Temperature: 28°C



References



Paw BH, Zon LI. Primary fibroblast cell culture.
   Methods Cell Biol. 59: 39-43, 1999. PubMed: 9891354




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