痛觉与感觉传导离子通道稳定表达细胞株BioVector®系列 / BioVector® Pain & Sensory Ion Channel Stable Expression Cell Line Series
- 价 格：¥998960
- 货 号：BioVector® PSICS
- 产 地：北京
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作微信:1843439339 (QQ同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
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BioVector® 痛觉与感觉传导离子通道稳定表达细胞株系列 / BioVector® Pain & Sensory Ion Channel Stable Expression Cell Line Series
一、 背景与产品线概览 / Background & Series Overview
BioVector® 痛觉与感觉传导离子通道稳定表达细胞株系列涵盖了外周与中枢感觉神经元（如背根神经节 DRG 神经元）中关键的TRP 瞬时受体电位通道（TRPA1、TRPV1/2/3、TRPM4/5/8）以及电压门控钠离子通道 Nav1.8。
该系列细胞株均以 BioVector® HEK293 或 BioVector® CHO-K1 为宿主细胞，采用高表达慢病毒或质粒载体构建，并经抗性（G418/Puromycin）筛选与单克隆克隆化，能够长期稳定表达高通量的活性通道蛋白。该系列是研究冷热刺激、机械痛觉、化学刺激（如辣椒素、芥末油、薄荷醇）、味觉传导及病理性疼痛（如神经病理性疼痛、炎症痛）机制，并进行高通量药物筛选（HTS）、钙荧光检测（FLIPR）和自动/手动膜片钳（APC）评估的金标准细胞模型。
二、 产品线核心靶点列表 / Target Specifications Table
| 产品名称 / Product Name | 基因/靶点 (Gene/Target) | 激活剂/激动剂 (Agonists) | 阻断剂/拮抗剂 (Antagonists) | 生理功能与应用 (Functions & Applications) |
| BioVector® TRPA1 稳定表达细胞株 | TRPA1 / TRPA1 | 芥末油 (AITC)、丙烯醛、冷刺激 ($<17^\circ\text{C}$) | HC-030031、A-967079 | 化学痛觉、冷痛觉及神经源性炎症 |
| BioVector® TRPV1 稳定表达细胞株 | TRPV1 / TRPV1 | 辣椒素 (Capsaicin)、热刺激 ($>43^\circ\text{C}$)、低 pH | Capsazepine、AMG-517 | 热痛觉敏化、炎症痛及镇痛药物筛选 |
| BioVector® TRPV2 稳定表达细胞株 | TRPV2 / TRPV2 | 极高热刺激 ($>52^\circ\text{C}$)、Probenecid、2-APB | Tranilast、Ruthenium Red | 高温痛觉、免疫巨噬细胞吞噬及心肌应激 |
| BioVector® TRPV3 稳定表达细胞株 | TRPV3 / TRPV3 | 温热刺激 ($33\text{--}39^\circ\text{C}$)、Carvacrol、Camphor | Formononetin、Isorhamnetin | 皮肤屏障、瘙痒感传导及表皮角质形成 |
| BioVector® TRPM4 稳定表达细胞株 | TRPM4 / TRPM4 | 胞内 $\text{Ca}^{2+}$ 升高（单价阳离子选择性） | 9-Phenanthrol、Glibenclamide | 细胞膜去极化、心律失常及缺血性脑损伤 |
| BioVector® TRPM5 稳定表达细胞株 | TRPM5 / TRPM5 | 胞内 $\text{Ca}^{2+}$ 升高（单价阳离子选择性） | Triphenylphosphine oxide (TPPO) | 甜/苦/鲜味觉传导、胰岛素分泌 |
| BioVector® TRPM8 稳定表达细胞株 | TRPM8 / TRPM8 | 薄荷醇 (Menthol)、WS-12、清凉刺激 ($<26^\circ\text{C}$) | AMTB、M8-B | 冷感受、冷痛觉及冷诱导的膀胱过度活动 |
| BioVector® Nav1.8 稳定表达细胞株 | SCN10A / $Na_V1.8$ | 电压去极化、TTX 耐受（TTX-R） | A-803467、VX-548 | DRG 痛觉动作电位产生、痛觉阻断剂筛选 |
三、 通用培养条件与传代指南 / Culture & Handling Protocols
1. 推荐培养基 / Complete Growth Medium
基础培养基：BioVector® High-Glucose DMEM (适用于 HEK293 宿主) 或 BioVector® DMEM/F-12 Medium (适用于 CHO-K1 宿主)
添加成分：10% 灭活 BioVector® Fetal Bovine Serum (FBS) + 1% 双抗（Penicillin-Streptomycin）
维持抗性：根据转染载体配比 BioVector® Geneticin (G418) (200–600 $\mu\text{g/mL}$) 或 BioVector® Puromycin (1–3 $\mu\text{g/mL}$)
培养环境：37 摄氏度，5% $\text{CO}_2$，高湿度培养箱
2. 复苏与传代操作 / Thawing & Subculture
复苏：将冻存管快速置于 37 摄氏度水浴中摇晃融化（1–2 分钟）。加入 8–10 mL 预热无抗性的 BioVector® Complete Growth Medium，120 x g 离心 5 分钟，弃上清后重悬接种于培养瓶中。贴壁恢复 24 小时后加入筛选抗性。
传代控制：
维持细胞密度在 70%–80% 时进行传代。
用无钙镁 DPBS 洗涤，加入 0.05% Trypsin-EDTA 消化 1–2 分钟，加入含血清培养基终止消化。
按 1:3 至 1:6 比例传代。
3. 冻存保存 / Cryopreservation
冻存液配方：90% BioVector® FBS + 10% DMSO 或 70% BioVector® Basal Medium + 20% BioVector® FBS + 10% DMSO
保存条件：程序降温后长期保存于液氮（-196 摄氏度）。
BioVector® Pain & Sensory Ion Channel Stable Expression Cell Line Series
1. Background and Series Overview
The BioVector® Pain and Sensory Ion Channel Stable Expression Series encompasses key Transient Receptor Potential (TRP) channels (TRPA1, TRPV1/2/3, TRPM4/5/8) and the voltage-gated sodium channel Nav1.8, which are highly expressed in primary sensory neurons (such as dorsal root ganglion, DRG neurons).
Utilizing BioVector® HEK293 or BioVector® CHO-K1 as host systems, these stable lines express functional ion channels generated via high-titer lentiviral or plasmid selection. They offer robust platforms for studying thermal, mechanical, chemical, and taste sensations, as well as developing therapeutic interventions for neuropathic and inflammatory pain via FLIPR calcium flux, high-throughput screening (HTS), and automated patch-clamp (APC) electrophysiology.
2. Target Specifications Table
| Product Name | Gene / Target | Canonical Agonists | Canonical Antagonists | Key Applications & Physiology |
| BioVector® TRPA1 Stable Cell Line | TRPA1 / TRPA1 | AITC (Mustard oil), Acrolein, Cold ($<17^\circ\text{C}$) | HC-030031, A-967079 | Chemical nociception, cold hyperalgesia, neurogenic inflammation |
| BioVector® TRPV1 Stable Cell Line | TRPV1 / TRPV1 | Capsaicin, Heat ($>43^\circ\text{C}$), Low pH | Capsazepine, AMG-517 | Thermal hyperalgesia, inflammatory pain, analgesic drug discovery |
| BioVector® TRPV2 Stable Cell Line | TRPV2 / TRPV2 | Extreme Heat ($>52^\circ\text{C}$), Probenecid, 2-APB | Tranilast, Ruthenium Red | High-threshold heat sensation, macrophage phagocytosis |
| BioVector® TRPV3 Stable Cell Line | TRPV3 / TRPV3 | Warmth ($33\text{--}39^\circ\text{C}$), Carvacrol, Camphor | Formononetin, Isorhamnetin | Skin barrier regulation, pruritus (itch) pathways |
| BioVector® TRPM4 Stable Cell Line | TRPM4 / TRPM4 | Intracellular $\text{Ca}^{2+}$ elevation (Monovalent-selective) | 9-Phenanthrol, Glibenclamide | Membrane depolarization, cardiac conduction, ischemic stroke |
| BioVector® TRPM5 Stable Cell Line | TRPM5 / TRPM5 | Intracellular $\text{Ca}^{2+}$ elevation (Monovalent-selective) | TPPO | Sweet/bitter/umami taste transduction, insulin secretion |
| BioVector® TRPM8 Stable Cell Line | TRPM8 / TRPM8 | Menthol, WS-12, Coolness ($<26^\circ\text{C}$) | AMTB, M8-B | Cold sensation, cold allodynia, bladder hyperreflexia |
| BioVector® Nav1.8 Stable Cell Line | SCN10A / $Na_V1.8$ | Voltage depolarization, TTX-Resistant (TTX-R) | A-803467, VX-548 | Nociceptive action potential firing, $Na_V1.8$ blocker screening |
3. Culture and Handling Protocols
Recommended Growth Medium
Basal Medium: BioVector® High-Glucose DMEM (for HEK293 host) or BioVector® DMEM/F-12 Medium (for CHO-K1 host)
Supplements: 10% heat-inactivated BioVector® Fetal Bovine Serum (FBS) + 1% Penicillin-Streptomycin
Selection Antibiotics: BioVector® Geneticin (G418) (200–600 $\mu\text{g/mL}$) or BioVector® Puromycin (1–3 $\mu\text{g/mL}$)
Culture Conditions: 37 degrees Celsius, 5% $\text{CO}_2$, humidified incubator
Subculture Protocol
Maintain working cell density at 70–80% confluency.
Wash with DPBS and treat with 0.05% Trypsin-EDTA for 1–2 minutes. Neutralize with serum-containing medium.
Split ratio: 1:3 to 1:6.
4. Technical Data Summary
| Parameter | Specification |
| Product Series | BioVector® 痛觉与感觉传导离子通道稳定表达细胞株系列 / BioVector® Pain & Sensory Ion Channel Series |
| Biosafety Level | BSL-1 |
| Host System | HEK293 / CHO-K1 |
| Assay Compatibilities | FLIPR Calcium Flux, Automated Patch Clamp (APC), Manual Patch Clamp, Binding Assays |
| Medium Formula | BioVector® High-Glucose DMEM (or BioVector® DMEM/F-12) + 10% BioVector® FBS + Selection Antibiotic |
BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心
电话:400-800-2947
工作QQ/微信同号:1843439339
E-mail:BioVector@163.com
网址http://www.biovector.net
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