hanbio H9c2(2-1) NONO KO大鼠自发永生化细胞系Rat Spontaneously immortalized cell line BioVector NTCC细胞保藏中心

[Designation名称] NTCC® hanbio H9c2(2-1) NONO KO Cell Line细胞株
BioVector NTCC典型培养物保藏中心

Comments: Characteristics: The knockout was carried out using pHBLV-U6-gRNA-EF1-Cas9-Puro lentivirus to infect the cells.
Comments: Knockout cell: Method=CRISPR/Cas9; RGD; 1549738; Nono.
Comments: Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
Comments: Derived from site: In situ; Embryonic heart, cardiac muscle; UBERON=UBERON_0003084+UBERON_0001133.
Comments: Breed/subspecies: BDIX; RS=RS_0000091.
Species of origin: NCBI_TaxID=10116; ! Rattus norvegicus (Rat)
Hierarchy: CVCL_0286 ! H9c2(2-1)
Sex of cell: Sex unspecified
Age of donor at sampling: Embryo
Category: Spontaneously immortalized cell line
Date (entry history): Created: 25-06-26; Last updated: 25-06-26; Version: 1
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[Organism物种来源]
[Disease疾病种类]
[编号]Accession Number: NTCC® CVCL_F4CM
[Comments描述]
[Reference参考文献]
[Category分类]
[Date保藏时间]
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培养方法：
Medium培养基：NTCC® hanbio H9c2(2-1) NONO KO Complete Medium
Growth properties形态：贴壁/悬浮：
Culture Condition培养条件：Temperature温度: 37°C.
Atmosphere: 95% Air, 5% CO2

Supplier生产厂家: BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
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