Cas9 RPE-1 VPS13C^mStayGold人类端粒酶永生化细胞系Human Telomerase immortalized cell line BioVector NTCC细胞保藏中心

[Designation名称] NTCC® Cas9 RPE-1 VPS13C^mStayGold Cell Line细胞株
BioVector NTCC典型培养物保藏中心

Synonyms: RPE-1 VPS13C^mStayGold
Comments: Characteristics: Stably expresses Streptococcus pyogenes Cas9 and overexpresses VPS13C internally tagged with the mStayGold fluorescent protein.
Comments: Genetic integration: Method=PiggyBac transposition; Gene=FPbase; 5H7QY; mStayGold.
Comments: Genetic integration: Method=PiggyBac transposition; Gene=HGNC; HGNC:23594; VPS13C.
Comments: Genetic integration: Method=PiggyBac transposition; Gene=UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR).
Comments: Genetic integration: Method=Transduction; Gene=UniProtKB; P0C2P0; Aspergillus terreus blasticidin-S deaminase (Bsd).
Comments: Genetic integration: Method=Transduction; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9.
Comments: Genetic integration: Method=Transfection; Gene=HGNC; HGNC:11730; TERT.
Comments: Genetic integration: Method=Transfection; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Comments: Miscellaneous: Cell line information from personal communication of Jessica, Eden.
Comments: Derived from site: In situ; Eye, retina, retinal pigment epithelium; UBERON=UBERON_0001782.
Comments: Cell type: Retinal pigment epithelial cell; CL=CL_0002586.
[Organism物种来源]
[Disease疾病种类]
[编号]Accession Number: NTCC® CVCL_F4CL
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[Reference参考文献]
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[Date保藏时间]
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培养方法：
Medium培养基：NTCC® Cas9 RPE-1 VPS13C^mStayGold Complete Medium
Growth properties形态：贴壁/悬浮：
Culture Condition培养条件：Temperature温度: 37°C.
Atmosphere: 95% Air, 5% CO2

Supplier生产厂家: BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
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