mND2-0 (MIRJT7i-mND2-0) 人诱导多能干细胞 / BioVector® mND2-0 Human Induced Pluripotent Stem Cell Line

BioVector® mND2-0 (MIRJT7i-mND2-0) 人诱导多能干细胞 / BioVector® mND2-0 Human Induced Pluripotent Stem Cell Line

通用定义 / General Definition:

BioVector® mND2-0（全称 MIRJT7i-mND2-0）是一种人源诱导多能干细胞 (iPSC) 系。该细胞系最初由威斯康星大学麦迪逊分校的 James Thomson 实验室建立，是利用非整合型质粒载体（如 Episomal vectors）转染人包皮成纤维细胞重编程而来的。mND2-0 是 NHLBI 祖细胞生物学联合会 (PCBC) 核心基因组库的重要组成部分。由于其具有高度的多能性和稳定的基因组背景，它被广泛用于人类器官构建、干细胞定向分化研究（如耳蜗、前庭和皮肤类器官）以及高通量基因组学分析。

BioVector® mND2-0 (also known as MIRJT7i-mND2-0) is a human induced pluripotent stem cell (iPSC) line. Originally derived in the laboratory of James Thomson at the University of Wisconsin-Madison, it was generated by reprogramming human foreskin fibroblasts using non-integrating episomal vectors. mND2-0 is a key reference line within the NHLBI Progenitor Cell Biology Consortium (PCBC) collection. Due to its robust pluripotency and well-characterized genomic profile, it is extensively utilized in the generation of complex human organoids (such as cochlear, vestibular, and hair-bearing skin organoids) and large-scale functional genomics studies.

BioVector® mND2-0 技术说明书 (Technical Datasheet)

中文版说明书 (Chinese Datasheet)

1. 产品基本信息

• 产品名称： BioVector® mND2-0 人诱导多能干细胞

• 来源组织： 人新生儿包皮成纤维细胞 (Foreskin Fibroblasts)

  
  

• 重编程方式： Episomal 质粒（含 OCT4, SOX2, NANOG, LIN28, c-MYC, KLF4）

• 生物特性： 多能性 (Pluripotency)，核型通常为正常 46, XY

• 应用领域： 类器官诱导（内耳、皮肤）、发育生物学模型、再生医学研究

2. 培养条件

• 基础培养基： BioVector® mTeSR Plus 或 TeSR-E8。

• 基质支持： 建议使用 Matrigel 或 Vitronectin 进行无滋养层培养。

• 培养环境： 37 摄氏度，5% CO2。

• 传代比例： 1:6 至 1:10；建议采用团块传代法（如 EDTA 或 ReLeSR）以维持干性。

3. 细胞应用

• 类器官建模： 近年来多篇顶级文献使用 mND2-0 成功诱导了具有功能性毛细胞的耳蜗类器官和带有毛囊的皮肤类器官。

• 基因组学对照： 作为 PCBC 计划的标准株，其全基因组测序、RNA-seq 及甲基化数据均可在公开数据库查阅。

• 分化能力： 可定向分化为神经元、心肌细胞及内皮细胞，用于疾病建模。

4. 注意事项

• 干性维持： iPSC 对培养基质量非常敏感。如果出现分化迹象（细胞边缘变得平滑或出现非典型形态），需及时手动剔除。

• 存储建议： 建议在液氮中长期保存，复苏时使用 Y-27632 (ROCK inhibitor) 以提高细胞存活率。

English Datasheet

1. General Product Information

• Product Name: BioVector® mND2-0 Human Induced Pluripotent Stem Cell Line

  
  

• Parental Source: Human neonatal foreskin fibroblasts

  
  

• Reprogramming Method: Non-integrating Episomal vectors (chemically defined conditions)

  
  

• Biological Properties: Pluripotent; characteristically exhibits a stable 46, XY karyotype.

• Primary Use: Organoid generation (inner ear, skin), developmental modeling, stem cell standardization.

2. Culture Conditions

• Basal Medium: BioVector® mTeSR Plus or TeSR-E8.

• Support Matrix: Feeder-free culture using Matrigel or recombinant Vitronectin.

• Incubation: 37°C, 5% CO2.

• Subculturing: Passage every 4-6 days (1:6 to 1:10 ratio) using clump-dissociation reagents such as EDTA.

3. Applications

• High-Fidelity Organoids: A preferred line for generating vestibular and cochlear organoids, as well as complex hair-bearing skin organoids.

• Reference Standards: Included in the PCBC genomic resource, providing a benchmark for SNP array, DNA methylation, and transcriptomics analyses.

• Lineage Specification: Ideal for studying the molecular switches governing the transition from pluripotency to ectodermal and mesodermal lineages.

4. Key Usage Notes

• Morphology Monitoring: Colonies should be tightly packed with well-defined borders. Spontaneous differentiation should be monitored and physically removed to ensure culture purity.

• Recovery: The use of a ROCK inhibitor (e.g., Y-27632) for the first 24 hours post-thawing is essential to maximize the recovery of high-quality pluripotent clusters.

注意 / Note: mND2-0 应在生物安全二级 (BSL-2) 条件下操作。使用前建议通过免疫荧光检测 OCT4, SSEA4 等标志物确认其多能状态。

mND2-0 should be handled under Biosafety Level 2 (BSL-2) conditions. BioVector® recommends verifying pluripotency markers such as OCT4 and SSEA4 via immunofluorescence before initiating large-scale differentiation protocols.

BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

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