BioVector® JVE253 人类黑素瘤细胞系 / BioVector® JVE253 Human Melanoma Cell Line

通用定义 / General Definition:BioVector® JVE253 是一种源自人类的黑素瘤 (Melanoma) 细胞系。该细胞系主要用于皮肤肿瘤学、癌症免疫学以及黑色素瘤生物学研究。JVE253 通常作为一种体外模型，用于探索黑色素瘤细胞的增殖、侵袭性、黑色素合成通路以及对各种化学疗法和靶向药物的敏感性。由于黑色素瘤具有高度的异质性，JVE253 为研究特定基因突变背景下的肿瘤进展提供了重要的细胞资源。

BioVector® JVE253 is a human-derived Melanoma cell line. This cell line is utilized primarily in dermatological oncology, cancer immunology, and basic melanoma biology research. JVE253 serves as an in vitro model for investigating melanoma cell proliferation, invasiveness, melanogenesis pathways, and sensitivity to various chemotherapeutic agents and targeted therapies. Given the high degree of heterogeneity in melanoma, JVE253 provides an important cellular resource for studying tumor progression within specific genetic mutation backgrounds.

BioVector® JVE253 技术说明书 (Technical Datasheet)

中文版说明书 (Chinese Datasheet)

1. 产品基本信息

• 产品名称: BioVector® JVE253 人黑色素瘤细胞

• 组织来源: 人类黑色素瘤组织

• 生长特性: 贴壁生长

• 细胞形态: 典型的人类黑色素瘤细胞形态，通常表现为梭形或多角形。

2. 培养条件

• 基础培养基: BioVector® RPMI 1640 或 DMEM（取决于具体亚株需求）。

• 血清添加: 10% BioVector® 优质胎牛血清 (FBS)。

• 补充因子: 建议添加 2 mM L-谷氨酰胺和 1% 青霉素-链霉素溶液。

• 培养环境: 37 摄氏度，5% CO2。

• 传代比例: 1:3 至 1:6，建议在汇合度达到 80% 到 90% 时进行传代。

3. 细胞应用

• 肿瘤生物学研究: 黑色素瘤细胞周期调控、凋亡诱导及细胞迁移能力测试。

• 药物筛选: 评估新型 BRAF 或 MEK 抑制剂等靶向药物的抗肿瘤活性。

• 免疫学研究: 研究黑色素瘤细胞与免疫细胞（如 T 细胞、NK 细胞）之间的相互作用。

4. 注意事项

• 黑色素沉积: 该细胞在培养过程中可能会产生不同程度的黑色素，这可能影响培养基的颜色，属于正常现象。

• 复苏建议: 细胞复苏后初期生长可能较慢，建议在复苏后的前 24 到 48 小时内不要频繁移动培养瓶。

English Datasheet

1. General Product Information

• Product Name: BioVector® JVE253 Human Melanoma Cell Line

• Source: Human melanoma tissue

• Growth Properties: Adherent

• Morphology: Characteristic melanoma morphology, typically appearing spindle-shaped or polygonal.

2. Culture Conditions

• Basal Medium: BioVector® RPMI 1640 or DMEM (depending on specific sub-line requirements).

• Serum Supplement: 10% BioVector® Fetal Bovine Serum (FBS).

• Supplements: 2 mM L-Glutamine and 1% Penicillin-Streptomycin are recommended.

• Incubation: 37 degrees Celsius, 5% CO2.

• Subculturing: 1:3 to 1:6 ratio; subculture when confluence reaches 80% to 90%.

3. Applications

• Cancer Research: Studying cell cycle regulation, apoptosis induction, and cell migration in melanoma.

• Drug Efficacy Assays: Testing the activity of novel targeted agents, such as BRAF or MEK inhibitors.

• Immune Interaction: Investigating the crosstalk between melanoma cells and immune effectors (e.g., T cells or NK cells).

4. Key Usage Notes

• Melanogenesis: The cells may produce varying levels of melanin during culture, which can darken the medium; this is a normal characteristic of this cell line.

• Thawing Procedure: Growth may be slow immediately after thawing. It is recommended to avoid excessive handling or observation during the first 24 to 48 hours post-recovery.

注意 / Note: JVE253 应在生物安全二级 (BSL-2) 条件下操作。在进行特定的分子实验前，建议先确认该细胞系的 BRAF, NRAS 等核心驱动基因的突变状态。JVE253 should be handled under Biosafety Level 2 (BSL-2) conditions. Before conducting specific molecular assays, it is recommended to verify the mutation status of key driver genes such as BRAF and NRAS for this cell line.

BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

电话:400-800-2947

工作QQ/微信同号:1843439339

网址

http://www.biovector.net