BioVector® PLum-AI 小鼠前列腺癌细胞系 / BioVector® PLum-AI Mouse Prostate Cancer Cell Line

通用定义 / General Definition:BioVector® PLum-AI 是一种源自转基因小鼠模型的人工工程化前列腺癌细胞系。该细胞系通常建立在 PTEN 缺失和 P53 缺失的遗传背景下，模拟了人类晚期前列腺癌最常见的基因改变。"AI" 通常代表 "Androgen Independent"（雄激素非依赖性） 或 "Androgen Insensitive"（雄激素不敏感），意味着该细胞系即使在低雄激素水平下也能持续生长。这使其成为研究去势抵抗性前列腺癌 (CRPC)、神经内分泌分化以及肿瘤干细胞特性的重要实验工具。

BioVector® PLum-AI is an engineered mouse prostate cancer cell line derived from transgenic mouse models. It is typically established on a genetic background of PTEN loss and P53 loss, mimicking the most frequent genomic alterations found in advanced human prostate cancer. The "AI" designation generally stands for "Androgen Independent" or "Androgen Insensitive", indicating the line's ability to proliferate despite low androgen levels. This makes it an essential experimental tool for studying Castration-Resistant Prostate Cancer (CRPC), neuroendocrine differentiation, and cancer stem cell characteristics.

BioVector® PLum-AI 技术说明书 (Technical Datasheet)

中文版说明书 (Chinese Datasheet)

1. 产品基本信息

• 产品名称： BioVector® PLum-AI 小鼠前列腺癌细胞

• 组织来源： 小鼠前列腺组织 (PTEN/P53 缺陷型)

• 分子特征：PTEN 缺失；P53 缺失；雄激素受体 (AR) 表达可能下调或发生功能转变；表现为去势抵抗特性。

• 生长特性： 贴壁生长。

• 细胞形态： 上皮样或纺锤形，具有较强的异质性，可见明显的恶性转化特征。

2. 培养条件

• 基础培养基： BioVector® PrEGM（前列腺上皮细胞生长培养基）或 DMEM/F-12 (1:1)。

• 血清添加： 5% - 10% BioVector® 优质胎牛血清 (FBS)（在研究雄激素不敏感性时，常使用活性炭/糊精处理的胎牛血清 CT-FBS）。

• 补充因子： 胰岛素、表皮生长因子 (EGF) 以及牛垂体提取物 (BPE)。

• 培养环境： 37 摄氏度，5% CO2。

• 传代比例： 1:3 至 1:6。

3. 细胞应用

• CRPC 机制研究： 探索前列腺癌如何从依赖雄激素状态演变为非依赖状态。

• 神经内分泌转分化： 研究 PTEN/P53 双缺失如何诱导肿瘤向恶性程度更高的神经内分泌表型转化。

• 同种异体移植模型： 由于其源自小鼠，可在具有免疫能力的同源小鼠（如 C57BL/6 背景）中接种，用于研究肿瘤免疫微环境。

4. 注意事项

• 血清敏感性： 雄激素水平对该细胞的表型维持至关重要。若要维持其“AI”特性，建议在无酚红培养基中配合 CT-FBS 培养。

• 接种密度： 细胞在低密度下可能生长较慢，建议保持适中的接种压力。

English Datasheet

1. General Product Information

• Product Name: BioVector® PLum-AI Mouse Prostate Cancer Cell Line

• Source: Mouse prostate tissue (PTEN/P53 deficient model)

• Molecular Profile:PTEN deletion; P53 deletion; Androgen Receptor (AR) signaling may be bypassed or altered; exhibits castration-resistant characteristics.

• Growth Properties: Adherent.

• Morphology: Epithelial-like or spindle-shaped; exhibits high heterogeneity and distinct features of malignant transformation.

2. Culture Conditions

• Basal Medium: BioVector® PrEGM (Prostate Epithelial Cell Growth Medium) or DMEM/F-12 (1:1).

• Serum Supplement: 5% - 10% BioVector® Fetal Bovine Serum (FBS). For studying androgen independence, Charcoal-Stripped FBS (CT-FBS) is strictly required.

• Supplements: Insulin, Epidermal Growth Factor (EGF), and Bovine Pituitary Extract (BPE).

• Incubation: 37°C, 5% CO2.

• Subculturing: 1:3 to 1:6 ratio.

3. Applications

• CRPC Mechanistic Studies: Investigating the molecular switch from androgen-dependent to androgen-independent growth.

• Neuroendocrine Differentiation (NED): Studying how the dual loss of PTEN and P53 drives the transdifferentiation into aggressive neuroendocrine phenotypes.

• Syngeneic Models: Being a mouse line, it can be implanted into immunocompetent mice (e.g., C57BL/6 background) to study tumor-immune system interactions.

4. Key Usage Notes

• Hormone Control: To accurately maintain and study the androgen-insensitive phenotype, avoid using phenol red-containing media and use charcoal-stripped serum to eliminate exogenous steroids.

• Phenotypic Stability: Regular monitoring of lineage markers (such as E-cadherin or Synaptophysin) is recommended to track potential neuroendocrine transdifferentiation during long-term culture.

注意 / Note: BioVector® PLum-AI 应在生物安全二级 (BSL-2) 条件下操作。由于该细胞系涉及复杂的转基因背景，建议在实验开始前通过 Western Blot 验证 PTEN 和 P53 的缺失状态。BioVector® PLum-AI should be handled under Biosafety Level 2 (BSL-2) conditions. Given the complex transgenic background of the line, BioVector® recommends verifying the PTEN and P53 status via Western Blot prior to initiating key experiments.

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