BioVector® PLum-AD 小鼠前列腺癌细胞系 / BioVector® PLum-AD Mouse Prostate Cancer Cell Line

通用定义 / General Definition:

BioVector® PLum-AD 是一种源自转基因小鼠模型的工程化前列腺癌细胞系。该细胞系与 PLum-AI 属于同一家族，通常建立在 PTEN 缺失和 P53 缺失的遗传背景下。"AD" 代表 "Androgen Dependent"（雄激素依赖型）。这意味着该细胞系的生长和存活高度依赖于雄激素信号通路。它是研究前列腺癌早期病变、雄激素受体（AR）信号转导以及探索从激素敏感性向激素非依赖性演变机制的关键对照模型。

BioVector® PLum-AD is an engineered mouse prostate cancer cell line derived from transgenic mouse models. Typically established on a PTEN-null and P53-null genetic background, the "AD" designation stands for "Androgen Dependent". This indicates that the proliferation and survival of this cell line are strictly dependent on androgen signaling. It serves as a pivotal model for studying early-stage prostate carcinogenesis, androgen receptor (AR) signal transduction, and the mechanisms underlying the transition from hormone-sensitive to hormone-refractory disease.

BioVector® PLum-AD 技术说明书 (Technical Datasheet)

中文版说明书 (Chinese Datasheet)

1. 产品基本信息

• 产品名称： BioVector® PLum-AD 小鼠前列腺癌细胞

• 组织来源： 小鼠前列腺组织 (PTEN/P53 缺陷型模型)

• 分子特征：PTEN 缺失；P53 缺失；维持高水平的雄激素受体 (AR) 表达及功能活性。

• 生长特性： 贴壁生长。

• 细胞形态： 典型的小鼠前列腺上皮样形态，排列较紧密。

2. 培养条件

• 基础培养基： BioVector® PrEGM 或 DMEM/F-12 (1:1)。

• 血清添加： 5% - 10% BioVector® 优质胎牛血清 (FBS)。

• 激素补充： 培养基中通常需要添加合成雄激素（如 1-10 nM R1881）以维持其雄激素依赖性表型。

• 培养环境： 37 摄氏度，5% $CO_2$。

• 传代比例： 1:3 至 1:5。

3. 细胞应用

• AR 信号研究： 研究雄激素与其受体结合后如何调控下游靶基因（如 PSA 同源物）的表达。

• 激素敏感性筛选： 评估新型抗雄激素药物（如恩杂鲁胺前体）的体外药效。

• 演变模型构建： 通过长期脱离雄激素培养，观察其向 PLum-AI 表型转化的过程。

4. 注意事项

• 雄激素维持： 若在不含雄激素的条件下（如使用 CT-FBS）培养，细胞生长会显著减缓并可能进入凋亡，因此必须确保培养体系中雄激素的稳定供应。

• 分化状态： 随着传代次数增加，细胞可能出现自发性的激素脱敏，建议在实验中使用低代数细胞。

English Datasheet

1. General Product Information

• Product Name: BioVector® PLum-AD Mouse Prostate Cancer Cell Line

• Source: Mouse prostate tissue (PTEN/P53 deficient model)

• Molecular Profile:PTEN deletion; P53 deletion; maintains robust Androgen Receptor (AR) expression and signaling activity.

• Growth Properties: Adherent.

• Morphology: Classic mouse prostatic epithelial morphology; cells tend to grow in organized clusters.

2. Culture Conditions

• Basal Medium: BioVector® PrEGM or DMEM/F-12 (1:1).

• Serum Supplement: 5% - 10% BioVector® Fetal Bovine Serum (FBS).

• Hormone Supplement: Requires supplementation with synthetic androgens (e.g., 1-10 nM R1881) to maintain the androgen-dependent growth phenotype.

• Incubation: 37°C, 5% $CO_2$.

• Subculturing: 1:3 to 1:5 ratio.

3. Applications

• AR Signaling Studies: Investigating the transcriptional regulation of downstream target genes following androgen binding.

• Hormone Sensitivity Assays: Evaluating the efficacy of novel anti-androgen compounds or AR degraders (PROTACs).

• Progression Modeling: Utilizing the line as a baseline to study the molecular triggers of castration resistance by inducing androgen deprivation in vitro.

4. Key Usage Notes

• Androgen Dependency: Proliferation is severely impaired in androgen-depleted media (e.g., media containing Charcoal-Stripped FBS without R1881). Consistent androgen levels are necessary for routine maintenance.

• Phenotypic Integrity: To avoid spontaneous drift toward an androgen-independent state, BioVector® recommends using low-passage cells and periodically verifying AR expression levels.

注意 / Note: BioVector® PLum-AD 应在生物安全二级 (BSL-2) 条件下操作。在进行涉及雄激素拮抗的实验时，建议同时使用 PLum-AI 作为阴性对照，以增强实验结论的准确性。

BioVector® PLum-AD should be handled under Biosafety Level 2 (BSL-2) conditions. When performing androgen antagonism assays, BioVector® recommends using the PLum-AI line as a negative control to ensure the specificity of the observed biological effects.

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