BioVector® AMPA-HEK293  AMPA受体稳定表达细胞系 / BioVector® AMPA Receptor Stable Expression Cell Line

通用定义 / General Definition:

BioVector® AMPA 表达细胞系（通常构建于 HEK293 或 CHO 细胞）是稳定转染了人源或大鼠源 AMPA 型谷氨酸受体（如 GluA1, GluA2, GluA3 或 GluA4 亚基）的工程化细胞。AMPA 受体是中枢神经系统中介导快速兴奋性突触传递的主要离子型受体。该细胞系通常还共表达辅助亚基（如 TARP $\gamma$-2 / Stargazin），以确保受体在细胞膜表面的正常定位和功能动力学。它是神经科学研究、电生理分析以及筛选抗癫痫药物和认知改善药物的核心工具。

BioVector® AMPA Expression Cell Lines (typically hosted in HEK293 or CHO cells) are engineered lines stably transfected with human or rat AMPA-type glutamate receptor subunits (e.g., GluA1, GluA2, GluA3, or GluA4). AMPA receptors are the primary ionotropic receptors mediating fast excitatory synaptic transmission in the central nervous system. These lines often co-express auxiliary subunits (such as TARP $\gamma$-2 / Stargazin) to ensure proper membrane trafficking and functional kinetics. They serve as essential tools for neuroscience research, electrophysiological profiling, and screening for anti-epileptic or pro-cognitive therapeutic candidates.

BioVector® AMPA 受体表达细胞系技术说明书 (Technical Datasheet)

中文版说明书 (Chinese Datasheet)

1. 产品基本信息

• 产品名称： BioVector® AMPA 受体 (GluA1-4) 稳定表达细胞系

• 母本细胞： HEK293 或 CHO-K1

• 表达靶点： 谷氨酸离子型受体 AMPA 型（可提供同源或异源四聚体组合）

• 生长特性： 贴壁生长

• 筛选抗生素： 含有 G418、潮霉素 (Hygromycin) 或 嘌呤霉素 (Puromycin)，用于维持受体复合体的稳定性。

2. 培养条件

• 基础培养基： BioVector® DMEM 或 F12 培养基。

• 血清添加： 10% BioVector® 优质胎牛血清 (FBS)。

• 关键注意事项： 培养基中可能需要添加谷氨酸受体拮抗剂（如 CNQX），以防止受体过度激活产生的兴奋性细胞毒性导致细胞死亡。

• 培养环境： 37 摄氏度，5% $CO_2$。

3. 细胞应用

• 高通量筛选 (HTS)： 利用荧光成像板读者 (FLIPR) 检测钙离子流或膜电位变化，筛选变构调节剂。

• 电生理研究： 利用全细胞膜片钳 (Patch Clamp) 研究受体的激活、脱敏及恢复动力学。

• 突触可塑性模拟： 研究受体亚基组成（如 GluA2 缺失导致的钙渗透性变化）对神经元功能的潜在影响。

4. 注意事项

• 受体脱敏： AMPA 受体脱敏极快（毫秒级）。在实验中通常需要添加脱敏抑制剂（如 Cyclothiazide / CTZ）来获得稳定的电流记录。

• 表达验证： 建议定期进行免疫荧光染色，确认受体在细胞表面的分布。

English Datasheet

1. General Product Information

• Product Name: BioVector® AMPA Receptor (GluA1-4) Stable Expression Cell Line

• Parental Line: HEK293 or CHO-K1

• Target Target: Glutamate Ionotropic Receptor AMPA Type (Available as homo- or hetero-tetrameric configurations)

• Growth Properties: Adherent

• Selection Markers: Maintained with G418, Hygromycin, or Puromycin to ensure stable maintenance of the receptor and auxiliary subunit plasmids.

2. Culture Conditions

• Basal Medium: BioVector® DMEM or F12 Medium.

• Serum Supplement: 10% BioVector® Fetal Bovine Serum (FBS).

• Key Requirement: Media may require supplementation with a non-competitive antagonist (e.g., CNQX) to prevent excitotoxicity-induced cell death caused by spontaneous receptor activation.

• Incubation: 37 degrees Celsius, 5% $CO_2$.

3. Applications

• High-Throughput Screening (HTS): Utilizing FLIPR assays for calcium flux or membrane potential changes to identify positive or negative allosteric modulators (PAMs/NAMs).

• Electrophysiology: Performing patch clamp recordings to characterize activation, deactivation, and desensitization kinetics.

• Drug Discovery: Evaluating novel compounds for the treatment of epilepsy, depression, or neurodegenerative diseases.

4. Key Usage Notes

• Desensitization Management: AMPA receptors exhibit extremely rapid desensitization. To obtain robust steady-state currents, the use of desensitization blockers like Cyclothiazide (CTZ) is often necessary during recordings.

• Passage Stability: Functional expression levels may decrease over time. BioVector® recommends verifying current density via patch clamp or ion imaging every 10 passages.

注意 / Note: BioVector® AMPA 受体表达细胞系应在生物安全二级 (BSL-2) 条件下操作。为获得最佳实验结果，请确保在电生理实验中使用快速灌流系统，以捕捉受体的瞬时峰值电流。

BioVector® AMPA expression cell lines should be handled under Biosafety Level 2 (BSL-2) conditions. For optimal results, BioVector® recommends using a fast-application perfusion system in electrophysiology to accurately capture transient peak currents.

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