BioVector® iPTi001-A 人诱导多能干细胞系 / BioVector® iPTi001-A Human Induced Pluripotent Stem Cell Line

通用定义 / General Definition:

BioVector® iPTi001-A 是一种标准化的人源诱导多能干细胞 (iPSC) 系，通常被记录为该系列中的首个参考株。该细胞系由健康供体或特定表型供体的体细胞（如外周血单核细胞或皮肤成纤维细胞）通过先进的非整合技术重编程而成。iPTi001-A 严格遵循多能性标准，具有卓越的自我更新能力和全向分化潜能。它是基础生物医学研究、干细胞库建设以及建立标准化分化方案（如向神经或心肌谱系分化）的基石模型。

BioVector® iPTi001-A is a standardized human Induced Pluripotent Stem Cell (iPSC) line, often designated as the primary reference strain within its series. This line is reprogrammed from somatic cells (e.g., PBMCs or skin fibroblasts) of a healthy or phenotypically characterized donor using advanced non-integrative technologies. iPTi001-A strictly adheres to pluripotency standards, exhibiting exceptional self-renewal capacity and omnipotent differentiation potential. It serves as a foundational model for basic biomedical research, stem cell banking, and the establishment of standardized differentiation protocols, such as neural or cardiomyocyte lineage specification.

BioVector® iPTi001-A 技术说明书 (Technical Datasheet)

中文版说明书 (Chinese Datasheet)

1. 产品基本信息

• 产品名称： BioVector® iPTi001-A 人 iPSC 细胞系

• 重编程平台： 非整合型（如塞内卡病毒或游离质粒），确保宿主基因组的原始性。

• 特性验证： * 标志物： 强表达 SSEA-4, TRA-1-60, TRA-1-81, OCT4 和 SOX2。

  • 三胚层能力： 已验证其在体内（畸胎瘤形成）或体外（胚状体 EB）形成三胚层的能力。

• 核型： 经鉴定为正常人类二倍体（46, XX 或 46, XY）。

2. 培养条件

• 维持培养基： BioVector® mTeSR Plus 或同级别无蛋白、无喂养层体系。

• 表面包被：Matrigel 或 Laminin-511。

• 培养环境： 37 摄氏度，5% $CO_2$。

• 传代建议： 采用 0.5 mM EDTA 进行集群传代（Clump passaging），每 4-6 天传代一次，比例为 1:6 至 1:12。

3. 细胞应用

• 发育模型： 研究人体从多能性状态向特定器官前体细胞转化的分子开关。

• 对照基准： 作为各种疾病特异性 iPSC 实验中的野生型（WT）对照株。

• 再生医学： 用于优化细胞大规模扩增工艺及临床前安全评估。

4. 注意事项

• 环境稳定性： iPSC 对 pH 值和渗透压变化极度敏感，建议每日固定时间换液。

• 存活增强： 在复苏或进行单细胞操作（如基因编辑后的克隆筛选）时，必须添加 10 $\mu$M ROCK 抑制剂 (Y-27632)。

English Datasheet

1. General Product Information

• Product Name: BioVector® iPTi001-A Human iPSC Line

• Reprogramming Platform: Non-integrative (e.g., Sendai virus or episomal vectors), ensuring no genomic scarring or integration.

• Characterization: * Markers: Robust expression of SSEA-4, TRA-1-60, TRA-1-81, OCT4, and SOX2.

  • Potency: Validated tri-lineage differentiation potential via in vivo (teratoma) or in vitro (embryoid body) assays.

• Karyotype: Confirmed normal human diploid karyotype.

2. Culture Conditions

• Maintenance Medium: BioVector® mTeSR Plus or equivalent feeder-free, chemically defined media.

• Coating Matrix:Matrigel or recombinant Laminin-511.

• Incubation: 37 degrees Celsius, 5% $CO_2$.

• Subculturing: Clump passaging using 0.5 mM EDTA is recommended; typical split ratios are 1:6 to 1:12 every 4–6 days.

3. Applications

• Developmental Modeling: Investigating molecular switches governing the transition from pluripotency to lineage-specific progenitor cells.

• Baseline Control: Utilized as a wild-type (WT) reference line in experiments involving disease-specific iPSC models.

• Regenerative Medicine: Optimizing large-scale cell expansion bioprocesses and performing pre-clinical safety assessments.

4. Key Usage Notes

• Environmental Consistency: iPSCs are highly sensitive to fluctuations in pH and osmolarity; BioVector® recommends media changes at a consistent time each day.

• Survival Enhancement: The addition of 10 $\mu$M ROCK inhibitor (Y-27632) is essential during thawing or single-cell procedures (e.g., clonal selection after gene editing) to prevent apoptosis.

注意 / Note: BioVector® iPTi001-A 应在生物安全二级 (BSL-2) 条件下操作。该细胞系经过多代验证，表现出极佳的遗传稳定性和分化效率。

BioVector® iPTi001-A should be handled under Biosafety Level 2 (BSL-2) conditions. This line has been validated across multiple passages, demonstrating superior genetic stability and differentiation efficiency.

BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

电话:400-800-2947

工作QQ/微信同号:1843439339

网址

http://www.biovector.net