BioVector® ZZUi0024-A 人诱导多能干细胞系 / BioVector® ZZUi0024-A Human Induced Pluripotent Stem Cell Line

通用定义 / General Definition:

BioVector® ZZUi0024-A 是一种人源诱导多能干细胞 (iPSC) 系，属于 ZZUi 标准化系列。该细胞系通常由特定临床特征供体（或作为对照的健康供体）的体细胞通过非整合型重编程技术建立。ZZUi0024-A 具备典型的干细胞生物学特性，包括分化为内、中、外三个胚层的能力以及在维持培养基中无限扩增的潜力。它是研究人类遗传学、发育生物学以及进行细胞替代疗法研究的重要工具。

BioVector® ZZUi0024-A is a human Induced Pluripotent Stem Cell (iPSC) line from the standardized ZZUi series. It is established from donor-derived somatic cells using non-integrative reprogramming technologies. ZZUi0024-A possesses classic biological hallmarks of stem cells, including the potential to differentiate into lineages of all three germ layers and the capacity for infinite expansion in maintenance media. It serves as a vital tool for human genetics, developmental biology, and cell-based regenerative medicine research.

BioVector® ZZUi0024-A 技术说明书 (Technical Datasheet)

中文版说明书 (Chinese Datasheet)

1. 产品基本信息

• 产品名称： BioVector® ZZUi0024-A 人 iPSC 细胞系

• 重编程载体： 非整合型（如塞内卡病毒或 Episomal 质粒），不改变宿主基因组结构。

• 多能性鉴定： * 免疫荧光： OCT4, SOX2, NANOG, SSEA-4 阳性。

  • 碱性磷酸酶 (AP)： 强阳性染色。

• 核型稳定性： 经 $G$-带扫描验证为正常二倍体。

2. 培养条件

• 维持体系： BioVector® mTeSR Plus 或 Essential 8 (E8) 培养基。

• 基质要求： 需包被 Matrigel 或 Vitronectin (玻连蛋白)。

• 培养环境： 37 摄氏度，5% $CO_2$。

• 传代比例： 1:6 至 1:10，建议采用 EDTA 或 ReLeSR 进行小集群传代。

3. 细胞应用

• 器官类器官构建： 诱导形成脑、肾或肠道类器官。

• 精准医疗： 结合基因编辑技术研究特定位点突变的致病性。

• 分化筛选： 研究小分子化合物对多能性维持或定向分化的影响。

4. 注意事项

• 自发分化： 定期检查集落。若出现非典型形态（如拉长、扁平细胞），应及时手动剔除。

• ROCK 抑制剂： 复苏及单细胞接种时，必须添加 10 $\mu$M Y-27632 以防止细胞凋亡。

English Datasheet

1. General Product Information

• Product Name: BioVector® ZZUi0024-A Human iPSC Line

• Reprogramming Vector: Non-integrative (e.g., Sendai virus or Episomal vectors), ensuring host genomic integrity.

• Pluripotency Validation: * Immunofluorescence: Positive for OCT4, SOX2, NANOG, and SSEA-4 markers.

  • Alkaline Phosphatase (AP): Strong positive staining.

• Karyotypic Stability: Confirmed normal diploid status via $G$-banding.

2. Culture Conditions

• Maintenance System: BioVector® mTeSR Plus or Essential 8 (E8) media.

• Coating Requirements: Plates must be pre-coated with Matrigel or Vitronectin.

• Incubation: 37 degrees Celsius, 5% $CO_2$.

• Subculturing: 1:6 to 1:10 ratio; non-enzymatic small clump passaging via EDTA or ReLeSR is recommended.

3. Applications

• Organoid Generation: Inducing the formation of brain, kidney, or intestinal 3D organoids.

• Precision Medicine: Combining with CRISPR/Cas9 to investigate the pathogenicity of specific genetic variants.

• Compound Screening: Studying the effects of small molecules on pluripotency maintenance or lineage-specific differentiation.

4. Key Usage Notes

• Monitoring Differentiation: Inspect colonies daily. Any atypical morphologies (e.g., elongated, fibroblastic cells) should be manually removed to preserve culture purity.

• Cell Survival: BioVector® recommends adding 10 $\mu$M ROCK inhibitor (Y-27632) during thawing and single-cell seeding to mitigate dissociation-induced apoptosis.

注意 / Note: BioVector® ZZUi0024-A 应在生物安全二级 (BSL-2) 条件下操作。该细胞系是研究人体细胞生物学和建立稳定分化协议的高效模型。

BioVector® ZZUi0024-A should be handled under Biosafety Level 2 (BSL-2) conditions. This cell line provides a robust model for human cell biology research and the establishment of stable differentiation protocols.

BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

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网址

http://www.biovector.net