BioVector® ZZUi0013-A 人诱导多能干细胞系 / BioVector® ZZUi0013-A Human Induced Pluripotent Stem Cell Line

通用定义 / General Definition:

BioVector® ZZUi0013-A 是一种标准化的人源诱导多能干细胞 (iPSC) 系。该细胞系通常是由特定临床背景的供体（或健康对照）的体细胞，通过先进的非整合重编程技术（如塞内卡病毒或游离质粒）重编程而成。ZZUi 系列通常与特定的医学研究中心或大学（如郑州大学，Zhengzhou University）相关联。ZZUi0013-A 具有典型的多能性特征，能够无限增殖并分化为所有三个胚层的细胞。它为研究人类疾病的发病机制、药物筛选及发育生物学提供了高质量的细胞模型。

BioVector® ZZUi0013-A is a standardized human Induced Pluripotent Stem Cell (iPSC) line. This line is established from donor-derived somatic cells using advanced non-integrative reprogramming technologies (such as Sendai virus or episomal vectors). The ZZUi series is typically associated with specific clinical cohorts or academic institutions (e.g., Zhengzhou University). ZZUi0013-A exhibits classic pluripotency characteristics, including the capacity for infinite self-renewal and the potential to differentiate into cells of all three germ layers. It serves as a high-quality cellular model for investigating human disease mechanisms, drug screening, and developmental biology.

BioVector® ZZUi0013-A 技术说明书 (Technical Datasheet)

中文版说明书 (Chinese Datasheet)

1. 产品基本信息

• 产品名称： BioVector® ZZUi0013-A 人 iPSC 细胞系

• 供体 background： 详细的供体临床背景（如特定疾病表型或健康对照）。

• 重编程方法： 非整合型（Non-integrative），确保基因组中不含有外源载体序列。

• 特性验证： * 标志物： 强表达 OCT4, NANOG, SOX2, SSEA4。

  • 核型： 具有正常的二倍体核型。

• 生长特性： 贴壁生长，集落边缘清晰。

2. 培养条件

• 维持培养基： BioVector® mTeSR Plus 或同级别无喂养层培养基。

• 基质包被：Matrigel 或 Laminin-511。

• 培养环境： 37 摄氏度，5% $CO_2$。

• 传代比例： 1:5 至 1:8，每 4-5 天传代一次。

3. 细胞应用

• 疾病建模： 若该系源自特定疾病患者，可用于模拟该疾病的细胞病理过程。

• 分化研究： 分化为功能性神经元、心肌细胞或类器官（Organoids）。

• 安全性筛选： 评估候选药物在人源背景下的潜在毒性。

4. 注意事项

• 细胞密度： 避免细胞过度汇合（Over-confluence），否则会增加自发分化的风险。

• 复苏建议： 复苏后前 24 小时建议使用含 ROCK 抑制剂 (Y-27632) 的培养基以提高存活率。

English Datasheet

1. General Product Information

• Product Name: BioVector® ZZUi0013-A Human iPSC Line

• Donor Profile: Detailed clinical background (e.g., specific disease phenotype or healthy control).

• Reprogramming Method: Non-integrative, ensuring the host genome remains free of vector integration.

• Characterization:

  • Markers: Strong expression of OCT4, NANOG, SOX2, and SSEA4.

  • Karyotype: Validated normal diploid karyotype.

• Growth Properties: Adherent; forms well-defined, compact colonies.

2. Culture Conditions

• Maintenance Medium: BioVector® mTeSR Plus or equivalent feeder-free system.

• Coating Substrate:Matrigel or Laminin-511.

• Incubation: 37 degrees Celsius, 5% $CO_2$.

• Subculturing: 1:5 to 1:8 ratio; typically passaged every 4–5 days.

3. Applications

• Disease Modeling: If derived from a specific patient, it serves to model the cytopathological processes of that disease.

• Lineage Differentiation: Differentiation into functional neurons, cardiomyocytes, or 3D organoids.

• Safety Pharmacology: Evaluating the toxicological profile of drug candidates in a human-specific genetic context.

4. Key Usage Notes

• Differentiation Control: Monitor the culture daily for spontaneous differentiation. Differentiated areas should be removed prior to passaging.

• Thawing Protocol: BioVector® recommends including a ROCK inhibitor (Y-27632) in the medium for the first 24 hours post-thaw to significantly improve cell attachment and viability.

注意 / Note: BioVector® ZZUi0013-A 应在生物安全二级 (BSL-2) 条件下操作。该细胞系对营养供应较为敏感，建议严格按照 BioVector® 推荐的换液频率进行维护。

BioVector® ZZUi0013-A should be handled under Biosafety Level 2 (BSL-2) conditions. The line is sensitive to nutrient fluctuations; BioVector® recommends strict adherence to the feeding schedule to maintain optimal pluripotency.

BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

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