BioVector® SPPHli004-A 人诱导多能干细胞系 / BioVector® SPPHli004-A Human Induced Pluripotent Stem Cell Line

通用定义 / General Definition:

BioVector® SPPHli004-A 是一种人源诱导多能干细胞 (iPSC) 系，它是 SPPHli 系列标准化细胞株中的重要成员。该细胞系由健康供体的体细胞通过非整合重编程技术（通常为塞内卡病毒或游离质粒）建立。SPPHli004-A 具有典型的人多能干细胞特征，能够无限自我更新，并在特定诱导条件下分化为体内任何类型的细胞。该细胞系经过严格的质量控制，包括核型分析、多能性标志物检测及三胚层分化能力验证，常作为生物医学研究中的标准对照株或分化研究的基础模型。

BioVector® SPPHli004-A is a human Induced Pluripotent Stem Cell (iPSC) line, a key member of the standardized SPPHli series. This line was established from somatic cells of a healthy donor using non-integrative reprogramming technologies (typically Sendai virus or episomal vectors). SPPHli004-A exhibits classic human pluripotent stem cell characteristics, capable of infinite self-renewal and differentiation into any cell type of the human body under specific inductive conditions. The line undergoes rigorous quality control, including karyotyping, pluripotency marker testing, and tri-lineage differentiation validation, serving as a standard control line or a foundational model for differentiation studies in biomedical research.

BioVector® SPPHli004-A 技术说明书 (Technical Datasheet)

中文版说明书 (Chinese Datasheet)

1. 产品基本信息

• 产品名称： BioVector® SPPHli004-A 人 iPSC 细胞系

• 供体背景： 健康供体，具有明确的性别及年龄背景资料。

• 重编程方式： 非整合型（Non-integrative），无病毒基因组残留。

• 质量鉴定： 已通过 $G$-带核型扫描（46, XX 或 46, XY）以及无支原体检测。

• 生长特性： 贴壁生长，在优质基质上形成边缘清晰、细胞致密的集落。

2. 培养条件

• 基础培养体系： BioVector® mTeSR Plus 或同类无蛋白/无喂养层培养基。

• 包被材料：Matrigel (基质胶) 或重组人 Laminin-511。

• 培养环境： 37 摄氏度，5% $CO_2$。

• 传代策略： 推荐使用 0.5 mM EDTA 进行离解，采用小集群传代（Small Clumps），传代比例通常为 1:6 至 1:10。

3. 细胞应用

• 遗传学研究： 作为对照组，评估疾病相关突变对细胞功能的影响。

• 细胞治疗探索： 研究多能干细胞向临床相关细胞类型（如心肌、神经、造血）的分化效率。

• 高通量筛选： 用于药物发现过程中早期的人源毒性测试和药效评估。

4. 注意事项

• 分化风险： 若集落中心出现空洞或边缘出现分化的成纤维样细胞，需立即进行手工清理（Scraping）。

• 储存： 长期储存于液氮气相（$-150$ 摄氏度以下）。

English Datasheet

1. General Product Information

• Product Name: BioVector® SPPHli004-A Human iPSC Line

• Donor Profile: Derived from a healthy donor; comprehensive demographic data available.

• Reprogramming Method: Non-integrative technology, ensuring the host genome remains free of vector sequences.

• Quality Control: Validated via $G$-banded karyotyping (46, XX or 46, XY) and mycoplasma-free certification.

• Growth Properties: Adherent; forms dense colonies with distinct borders on high-quality substrates.

2. Culture Conditions

• Culture System: BioVector® mTeSR Plus or equivalent protein-free/feeder-free media.

• Coating Substrate:Matrigel or recombinant human Laminin-511.

• Incubation: 37 degrees Celsius, 5% $CO_2$.

• Subculturing: Recommended dissociation using 0.5 mM EDTA for small clump passaging; typical split ratios range from 1:6 to 1:10.

3. Applications

• Genetic Research: Serving as a baseline control to evaluate the impact of disease-related mutations on cellular phenotypes.

• Cell Therapy Development: Investigating the differentiation efficiency into clinically relevant lineages (e.g., cardiomyocytes, neurons, hematopoietic cells).

• High-Throughput Screening: Utilized for early-stage human-derived toxicity testing and efficacy evaluation in drug discovery.

4. Key Usage Notes

• Differentiation Management: Monitor daily. If hollow centers or fibroblastic-like cells appear at colony edges, perform manual scraping to maintain culture purity.

• Cryopreservation: Store in the vapor phase of liquid nitrogen (below $-150$ degrees Celsius) for long-term viability.

注意 / Note: BioVector® SPPHli004-A 应在生物安全二级 (BSL-2) 条件下操作。该细胞系是构建等基因对照 (Isogenic Controls) 的理想母本。在进行基因组编辑时，建议配合 BioVector® 专用转染试剂盒以提高成功率。

BioVector® SPPHli004-A should be handled under Biosafety Level 2 (BSL-2) conditions. This line is an ideal parental candidate for generating isogenic controls. For genomic editing, BioVector® recommends using specialized transfection kits to optimize efficiency.

BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

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网址

http://www.biovector.net