BioVector® SPPHli003-A 人诱导多能干细胞系 / BioVector® SPPHli003-A Human Induced Pluripotent Stem Cell Line

通用定义 / General Definition:

BioVector® SPPHli003-A 是一种标准化的人源诱导多能干细胞 (iPSC) 系。该细胞系通常由健康供体的体细胞（如外周血单核细胞 PBMC 或皮肤成纤维细胞）通过非整合型重编程技术（如塞内卡病毒或游离质粒）制备而成。SPPHli003-A 被广泛用作正常对照株 (Control Line)，用于与携带特定疾病突变的 iPSC 进行比较研究。它具有自我更新能力和分化为内、中、外三个胚层所有细胞类型的潜能。

BioVector® SPPHli003-A is a standardized human Induced Pluripotent Stem Cell (iPSC) line. This line is typically reprogrammed from somatic cells (such as PBMCs or skin fibroblasts) of a healthy donor using non-integrative technologies (e.g., Sendai virus or episomal vectors). SPPHli003-A is widely utilized as a normal control line for comparative studies with disease-specific iPSC models. It possesses the capacity for infinite self-renewal and the potential to differentiate into all cell types of the three germ layers (ectoderm, mesoderm, and endoderm).

BioVector® SPPHli003-A 技术说明书 (Technical Datasheet)

中文版说明书 (Chinese Datasheet)

1. 产品基本信息

• 产品名称： BioVector® SPPHli003-A 人 iPSC 细胞系

• 供体背景： 健康供体，通常包含完整的临床背景及人口统计学资料。

• 重编程方式： 非整合型技术，确保宿主基因组无外源基因残留。

• 多能性标志物： 高表达 OCT4, NANOG, SOX2, SSEA4, TRA-1-60 及 TRA-1-81。

• 生长特性： 贴壁生长，呈致密的集落状（Colony）。

2. 培养条件

• 维持培养基： BioVector® mTeSR Plus 或 mTeSR1 无喂养层培养体系。

• 包被基质： 必须使用 基质胶 (Matrigel) 或 Laminin-511 进行预包被。

• 培养环境： 37 摄氏度，5% $CO_2$。

• 传代方式： 建议使用 EDTA 或 ReLeSR 进行非酶类成簇传代，以保持细胞干性。

3. 细胞应用

• 对照研究： 作为基因编辑实验（如 CRISPR/Cas9）的母本细胞或疾病模型的野生型对照。

• 发育生物学： 研究人类早期胚胎发育过程。

• 药物毒性评估： 分化为心肌细胞、肝细胞或神经元，用于评估药物的早期安全性。

4. 注意事项

• 自发分化： 需每日观察。若集落边缘出现扁平、散乱的细胞，说明发生自发分化，需及时清除。

• 核型监测： 建议每 10-15 代进行一次 G 带核型分析，以确保染色体结构正常。

English Datasheet

1. General Product Information

• Product Name: BioVector® SPPHli003-A Human iPSC Line

• Donor Profile: Derived from a healthy donor; comprehensive clinical and demographic data are typically available.

• Reprogramming Method: Non-integrative methods to ensure no exogenous DNA integration into the host genome.

• Pluripotency Markers: Strong expression of OCT4, NANOG, SOX2, SSEA4, TRA-1-60, and TRA-1-81.

• Growth Properties: Adherent; grows in dense, well-defined colonies.

2. Culture Conditions

• Maintenance Medium: BioVector® mTeSR Plus or mTeSR1 (Feeder-free system).

• Surface Coating: Plates must be pre-coated with Matrigel or Laminin-511.

• Incubation: 37 degrees Celsius, 5% $CO_2$.

• Subculturing: Non-enzymatic clump passaging using EDTA or ReLeSR is highly recommended to maintain pluripotency.

3. Applications

• Control Studies: Serving as the parental line for gene editing (e.g., CRISPR/Cas9) or as a wild-type control for disease-specific iPSC models.

• Developmental Biology: Modeling early human embryonic development and lineage specification.

• Drug Toxicology: Differentiation into specialized cells (cardiomyocytes, hepatocytes, or neurons) for early-stage drug safety screening.

4. Key Usage Notes

• Spontaneous Differentiation: Monitor daily. Differentiated cells (characterized by flattened or enlarged morphology at colony edges) should be mechanically removed or avoided during passaging.

• Genomic Integrity: BioVector® recommends routine G-banded karyotyping every 10–15 passages to verify chromosomal stability.

注意 / Note: BioVector® SPPHli003-A 应在生物安全二级 (BSL-2) 条件下操作。该细胞系对操作者的传代技术要求较高，建议在实验前进行标准化培训以确保集落质量。

BioVector® SPPHli003-A should be handled under Biosafety Level 2 (BSL-2) conditions. This cell line requires high proficiency in passaging techniques; BioVector® suggests standardized training to ensure the maintenance of high-quality pluripotent colonies.

BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

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网址

http://www.biovector.net