BioVector® BL-60 人鼻咽癌细胞系 / BL-60 Human Nasopharyngeal Carcinoma Cell Line

通用定义 / General Definition:BioVector® BL-60 是一种源自人类鼻咽癌 (Nasopharyngeal Carcinoma, NPC) 的上皮细胞系。与 BL-89 类似，它是研究鼻咽恶性肿瘤的重要实验工具。该细胞系在体外表现出稳定的生长特性，被广泛应用于基础肿瘤生物学研究，包括细胞增殖动力学、肿瘤相关抗原的表达、以及对各种抗癌活性成分（如化疗药物、合成小分子或植物提取物）的细胞毒性评价。

BioVector® BL-60 is an epithelial cell line derived from Human Nasopharyngeal Carcinoma (NPC). Similar to BL-89, it serves as an important experimental tool for studying nasopharyngeal malignancies. This cell line exhibits stable growth characteristics in vitro and is widely used in basic tumor biology research, including cell proliferation kinetics, the expression of tumor-associated antigens, and cytotoxicity evaluations of various anti-cancer active ingredients (such as chemotherapeutics, synthetic small molecules, or plant extracts).

BioVector® BL-60 技术说明书 (Technical Datasheet)

中文版说明书

1. 产品基本信息

• 产品名称： BioVector® BL-60 人鼻咽癌细胞

• 组织来源： 鼻咽 (Nasopharynx)

• 疾病背景： 鼻咽癌 (NPC)

• 生长特性： 贴壁生长

• 细胞形态： 上皮样 (Epithelial-like)

2. 培养条件

• 基础培养基： BioVector® RPMI-1640 或 DMEM 培养基

• 血清添加： 10% BioVector® 优质胎牛血清 (FBS)

• 培养环境： 37 摄氏度，5% CO2，恒温加湿

• 传代比例： 1:3 至 1:5；建议在细胞汇合度达到 85% 左右时传代。

3. 细胞应用

• 抗癌药物筛选： 建立体外模型筛选具有潜在抗 NPC 活性的候选药物。

• 生物标志物探索： 研究 NPC 细胞表面的特异性受体或胞内信号分子。

• 细胞死亡机制： 观察药物诱导的凋亡、坏死或自噬过程。

4. 注意事项

• 支原体预防： 建议每两周进行一次支原体检测，确保培养体系的洁净。

• 细胞异质性： 在长期传代过程中，细胞表型可能发生微小漂移，建议在实验中使用低代数细胞。

English Datasheet

1. General Product Information

• Product Name: BioVector® BL-60 Human Nasopharyngeal Carcinoma Cell Line

• Tissue Source: Nasopharynx

• Disease Context: Nasopharyngeal Carcinoma (NPC)

• Growth Properties: Adherent

• Morphology: Epithelial-like

2. Culture Conditions

• Basal Medium: BioVector® RPMI-1640 or DMEM Medium

• Serum Supplement: 10% BioVector® Fetal Bovine Serum (FBS)

• Incubation: 37 degrees Celsius, 5% CO2, Humidified Atmosphere

• Subculturing: 1:3 to 1:5 ratio; subculture when cells reach approximately 85% confluence.

3. Applications

• Anti-cancer Drug Screening: Establishing in vitro models to screen drug candidates with potential anti-NPC activity.

• Biomarker Exploration: Investigating specific cell surface receptors or intracellular signaling molecules in NPC cells.

• Cell Death Mechanisms: Observing drug-induced apoptosis, necrosis, or autophagy processes.

4. Key Usage Notes

• Mycoplasma Prevention: Periodic mycoplasma testing (e.g., every two weeks) is recommended to ensure the purity of the culture system.

• Phenotypic Stability: To minimize genetic and phenotypic drift associated with long-term culture, BioVector® recommends using low-passage cells for definitive experiments.

Note: BL-60 cells should be handled under Biosafety Level 2 (BSL-2) protocols. For consistent experimental outcomes, BioVector® suggests the use of its validated high-purity FBS and standardized RPMI-1640 medium.

BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

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