BioVector® BL-89 人鼻咽癌细胞系 / BL-89 Human Nasopharyngeal Carcinoma Cell Line

通用定义 / General Definition:BioVector® BL-89 是一种源自人类鼻咽癌 (Nasopharyngeal Carcinoma, NPC) 的上皮细胞系。鼻咽癌在组织学上通常分为角化性和非角化性，而 BL-89 常被用作研究鼻咽癌恶性演变、肿瘤免疫微环境以及病毒相关发病机制（如 EB 病毒的影响）的体外模型。该细胞系具有较强的增殖能力和成瘤性，是头颈部肿瘤学研究中不可或缺的工具。

BioVector® BL-89 is an epithelial cell line derived from Human Nasopharyngeal Carcinoma (NPC). NPC is histologically categorized into keratinizing and non-keratinizing types; BL-89 is frequently utilized as an in vitro model to study malignant progression, the tumor immune microenvironment, and virus-related pathogenesis (such as the influence of the Epstein-Barr Virus). Characterized by its robust proliferative capacity and tumorigenicity, this cell line is an essential tool in head and neck oncology research.

BioVector® BL-89 技术说明书 (Technical Datasheet)

中文版说明书

1. 产品基本信息

• 产品名称： BioVector® BL-89 人鼻咽癌细胞

• 组织来源： 鼻咽 (Nasopharynx)

• 疾病背景： 鼻咽癌 (NPC)

• 生长特性： 贴壁生长

• 细胞形态： 上皮样 (Epithelial-like)，呈多角形，贴壁紧密

2. 培养条件

• 基础培养基： BioVector® RPMI-1640 或 DMEM 培养基

• 血清添加： 10% BioVector® 优质胎牛血清 (FBS)

• 培养环境： 37 摄氏度，5% CO2，恒温加湿

• 传代比例： 1:3 至 1:5；建议在细胞汇合度达到 80%-90% 时进行传代。

3. 细胞应用

• 致癌机制研究： 探索调控 NPC 细胞周期、凋亡及自噬的分子通路。

• 药物耐受性分析： 建立针对顺铂、 5-氟尿嘧啶等常用化疗药的耐药模型。

• 侵袭与转移： 研究上皮-间充质转化 (EMT) 在鼻咽癌扩散中的作用。

4. 注意事项

• 身份鉴定： 建议实验室定期进行 STR 鉴定，以区分 BL-89 与其他相似的 NPC 细胞系（如 CNE 系列）。

• 细胞冻存： 建议使用 BioVector® 专用无血清细胞冻存液，以确保复苏后的高活力。

English Datasheet

1. General Product Information

• Product Name: BioVector® BL-89 Human Nasopharyngeal Carcinoma Cell Line

• Tissue Source: Nasopharynx

• Disease Context: Nasopharyngeal Carcinoma (NPC)

• Growth Properties: Adherent

• Morphology: Epithelial-like, polygonal, tightly adherent

2. Culture Conditions

• Basal Medium: BioVector® RPMI-1640 or DMEM Medium

• Serum Supplement: 10% BioVector® Fetal Bovine Serum (FBS)

• Incubation: 37 degrees Celsius, 5% CO2, Humidified Atmosphere

• Subculturing: 1:3 to 1:5 ratio; subculture when cells reach 80%-90% confluence.

3. Applications

• Oncogenesis Mechanisms: Exploring molecular pathways regulating the cell cycle, apoptosis, and autophagy in NPC cells.

• Drug Resistance Analysis: Establishing models for resistance against common chemotherapeutics like Cisplatin or 5-Fluorouracil.

• Invasion and Metastasis: Investigating the role of Epithelial-Mesenchymal Transition (EMT) in the dissemination of nasopharyngeal cancer.

4. Key Usage Notes

• Authentication: Labs are encouraged to perform regular STR profiling to distinguish BL-89 from other similar NPC lines (e.g., the CNE series).

• Cryopreservation: BioVector® recommends using specialized serum-free freezing media to ensure maximum viability upon recovery.

Note: BL-89 cells should be handled under Biosafety Level 2 (BSL-2) protocols. For consistent results in signaling studies, BioVector® recommends using validated FBS and avoiding over-confluence during routine maintenance.

BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

电话:400-800-2947

工作QQ/微信同号:1843439339

网址

http://www.biovector.net