BioVector® mHypoE-N1 小鼠下丘脑神经元细胞系 / mHypoE-N1 Mouse Hypothalamic Neuronal Cell Line

通用定义 / General Definition:BioVector® mHypoE-N1 是一种源自成年小鼠下丘脑的永生化神经元细胞系。该细胞系通过逆转录病毒介导的 SV40 T-antigen 转化技术建立。mHypoE-N1 保持了下丘脑神经元的许多关键生理特征，包括对各种激素（如胰岛素、瘦素）和神经递质的反应能力。它是研究神经内分泌调控、能量代谢平衡、昼夜节律以及下丘脑信号转导机制的理想体外模型。

BioVector® mHypoE-N1 is an immortalized neuronal cell line derived from the adult mouse hypothalamus. This cell line was established using retroviral-mediated SV40 T-antigen transformation. mHypoE-N1 cells retain many key physiological characteristics of hypothalamic neurons, including responsiveness to various hormones (such as insulin and leptin) and neurotransmitters. It serves as an ideal in vitro model for studying neuroendocrine regulation, energy homeostasis, circadian rhythms, and hypothalamic signaling mechanisms.

BioVector® mHypoE-N1 技术说明书 (Technical Datasheet)

中文版说明书

1. 产品基本信息

• 产品名称： BioVector® mHypoE-N1 小鼠下丘脑神经元细胞

• 细胞来源： 小鼠 (Mouse / Mus musculus)

• 组织来源： 下丘脑 (Hypothalamus)

• 生长特性： 贴壁生长

• 细胞形态： 神经元样（具有胞体和轴突样突起）

2. 培养条件

• 基础培养基： BioVector® 高糖 DMEM 培养基

• 血清添加： 10% BioVector® 优质胎牛血清 (FBS)

• 培养环境： 37 摄氏度，5% CO2

• 传代比例： 1:3 至 1:5；建议在细胞达到 80% 汇合时进行传代，避免细胞过密导致分化状态改变。

3. 细胞应用

• 代谢调节研究： 研究瘦素 (Leptin) 和胰岛素 (Insulin) 信号通路对神经元的影响。

• 神经内分泌学： 探索下丘脑激素的合成、分泌及其调节机制。

• 筛选实验： 评估穿透血脑屏障的药物对中枢神经系统的潜在作用。

4. 注意事项

• 细胞状态： 神经元细胞系对培养环境较为敏感，请确保使用预热的培养基并轻柔操作。

• 鉴定验证： 建议定期检测神经元标志物（如截短型神经丝蛋白或特定的下丘脑神经肽基因表达）以确认细胞表型。

English Datasheet

1. General Product Information

• Product Name: BioVector® mHypoE-N1 Mouse Hypothalamic Neuronal Cell Line

• Organism: Mouse (Mus musculus)

• Tissue Source: Hypothalamus

• Growth Properties: Adherent

• Morphology: Neuronal (exhibiting cell bodies and neurite-like processes)

2. Culture Conditions

• Basal Medium: BioVector® High-glucose DMEM Medium

• Serum Supplement: 10% BioVector® Fetal Bovine Serum (FBS)

• Incubation: 37 degrees Celsius, 5% CO2

• Subculturing: 1:3 to 1:5 ratio; it is recommended to subculture when cells reach 80% confluence to prevent changes in differentiation state due to overcrowding.

3. Applications

• Metabolic Regulation: Investigating the impact of Leptin and Insulin signaling pathways on neuronal function.

• Neuroendocrinology: Exploring the mechanisms governing the synthesis, secretion, and regulation of hypothalamic hormones.

• Screening Assays: Evaluating the central nervous system effects of drugs designed to cross the blood-brain barrier.

4. Key Usage Notes

• Culture Sensitivity: Neuronal cell lines are sensitive to environmental shifts. Always use pre-warmed media and handle the cells gently during dissociation.

• Phenotypic Validation: Periodically monitoring neuronal markers or the expression of specific hypothalamic neuropeptide genes is recommended to ensure the functional integrity of the BioVector® mHypoE-N1 line.

Note: For experiments requiring high transfection efficiency, BioVector® recommends using specialized neuronal transfection reagents. To maintain the long-term stability of the cell line, ensure the use of BioVector® validated FBS and minimize the number of passages.

Supplier生产厂家: BioVector NTCC典型培养物保藏中心

E-mail: BioVector@163.com

http://www.biovector.net