BioVector® Caco-2 BBE 人类结肠腺癌细胞系 / Caco-2BBE Human Colorectal Adenocarcinoma Cell Line

通用定义 / General Definition:

BioVector® Caco-2BBE (C2BBe1) 是从 Caco-2 细胞系中通过极限稀释法筛选出的一个亚克隆。相比于原始的 Caco-2，BioVector® Caco-2BBE 在成层、极化以及刷状缘（Brush Border）蛋白（如蔗糖酶-异构麦芽糖酶）的表达上表现出更高的一致性和形态学上的优越性。它能够形成极其紧密的单层细胞膜，是目前模拟人小肠上皮屏障、研究微绒毛结构、营养吸收以及药物渗透性（如 Caco-2 渗透实验）的国际公认标准模型。

BioVector® Caco-2BBE (C2BBe1) is a subclone isolated from the Caco-2 cell line via limiting dilution. Compared to parental Caco-2, BioVector® Caco-2BBE exhibits superior homogeneity in stratification, polarization, and the expression of brush border enzymes (e.g., sucrase-isomaltase). It forms highly organized, confluent monolayers that more closely resemble the human intestinal epithelium. It is the gold-standard model for studying microvilli structure, nutrient absorption, and drug permeability (e.g., Caco-2 permeability assays).

BioVector® Caco-2BBE 细胞系技术说明书 (Cell Line Datasheet)

中文版说明书

1. 产品基本信息

• 产品名称： BioVector® Caco-2BBE 人结肠癌细胞（刷状缘表达克隆）

• 细胞来源： 人类 (Human)

• 组织来源： 结肠/直肠 (Colon/Rectum)

• 生长特性： 贴壁生长

• 细胞形态： 上皮细胞样

2. 培养条件

• 基础培养基： BioVector® DMEM 培养基（含 4.5 g/L 葡萄糖）

• 血清添加： 10% BioVector® 胎牛血清 (FBS)

• 添加剂： 0.01 mg/mL BioVector® 人转铁蛋白 (Human Transferrin)

• 培养环境： 37°C，5% $CO_2$，饱和湿度

3. 细胞应用

• 药物渗透性实验： 测量药物穿过肠道单层的转运效率（Papp 值）。

• 细胞极性研究： 研究顶端 (Apical) 与基底侧 (Basolateral) 的蛋白定位。

• 肠道致病菌感染模型： 研究细菌对肠道屏障的黏附与侵袭。

4. 注意事项

• 分化周期： 细胞在达到 100% 汇合后需要额外的 14-21 天才能完全极化和分化。

• 汇合度管理： 传代时应避免细胞长期处于汇合状态，否则会引起过早分化，导致实验结果不稳定。建议在 80% 汇合时传代。

English Datasheet

1. General Product Information

• Product Name: BioVector® Caco-2BBE Human Colorectal Adenocarcinoma Cell Line

• Organism: Human

• Tissue Source: Colon / Rectum

• Growth Properties: Adherent

• Morphology: Epithelial-like

2. Culture Conditions

• Basal Medium: BioVector® DMEM Medium (4.5 g/L Glucose)

• Serum Supplement: 10% BioVector® Fetal Bovine Serum (FBS)

• Supplement: 0.01 mg/mL BioVector® Human Transferrin

• Incubation: 37°C, 5% $CO_2$, Saturated Humidity

3. Applications

• Drug Permeability Assays: Measuring the transport efficiency (Papp values) of compounds across the intestinal monolayer.

• Cell Polarity Studies: Investigating protein localization between apical and basolateral membranes.

• Enteric Infection Models: Studying the adhesion and invasion of intestinal pathogens.

4. Key Usage Notes

• Differentiation Period: Cells require an additional 14-21 days post-confluence to achieve full polarization and brush border enzyme maturation.

• Confluence Management: To maintain the proliferative capacity of the stock, do not allow cells to remain confluent for extended periods before subculturing. Subculture is recommended at 80% confluence.

Note: Handle BioVector® Caco-2BBE cells under Biosafety Level 2 (BSL-2) conditions. For consistent results in permeability assays, the use of BioVector® DMEM Medium and BioVector® Fetal Bovine Serum is critical to maintain tight junction integrity (TER values).

Supplier生产厂家: BioVector NTCC典型培养物保藏中心

E-mail: BioVector@163.com

http://www.biovector.net