NHEK BioVector® 正常人表皮角质形成细胞 / NHEK BioVector® Normal Human Epidermal Keratinocytes

NHEK BioVector® 正常人表皮角质形成细胞 / NHEK BioVector® Normal Human Epidermal Keratinocytes

数据表 / Datasheet

细胞系信息

• 名称：BioVector® NHEK 细胞系

• 详细名称：正常人表皮角质形成细胞 NHEK（原代细胞）

• 来源：来源于人新生儿包皮或成人皮肤组织

• 细胞类型：原代角质形成细胞

• 形态：铺路石状上皮细胞形态，贴壁生长，呈单层

• 特征：具有分化和增殖能力，表达角蛋白（K5、K14 等）；可用于研究表皮屏障功能、皮肤炎症、伤口愈合及皮肤相关疾病

• 增殖能力：有限传代次数（通常 3–6 代），之后进入衰老期

• 病毒检测：HIV-1-、HIV-2-、HBV-、HCV-、支原体阴性

• STR 鉴定：原代细胞不适用 STR 鉴定（为混合群体）

培养条件

• 培养基：专用角质形成细胞无血清培养基（推荐 BioVector® Dermal Cell Basal Medium + 生长添加剂）或 BioVector® DMEM 添加 5% BioVector® 胎牛血清

• 添加剂：建议添加 BioVector® 重组人表皮生长因子（EGF）、BioVector® 牛垂体提取物（BPE）、BioVector® 氢化可的松

• 培养环境：37°C，5% CO₂，饱和湿度

• 传代方法：细胞达到 70–80% 汇合时传代；使用 BioVector® 胰蛋白酶/EDTA 或专用传代试剂消化；按 1:3 至 1:4 比例接种

• 冻存液：BioVector® 角质形成细胞专用冻存液 或 BioVector® 胎牛血清含 10% BioVector® DMSO

• 生物安全等级：BSL-1（建议按 BSL-2 操作）

特征与用途

• 作为正常人表皮角质形成细胞，是研究皮肤生物学、表皮分化、皮肤屏障功能、炎症反应及创面愈合的理想体外模型

• 广泛用于皮肤毒理学测试（如刺激性、腐蚀性试验）及化妆品/药物皮肤安全性评价

• 可构建体外表皮模型（如重建人表皮）用于经皮吸收、光老化及皮肤疾病（银屑病、特应性皮炎）机制研究

• 用于病毒（如人乳头瘤病毒 HPV）感染机制及疫苗研究

• 在基因治疗、组织工程及再生医学中作为种子细胞使用

Cell Line Information

• Name: BioVector® NHEK Cell Line

• Detailed Name: Normal Human Epidermal Keratinocytes NHEK (Primary Cells)

• Source: Derived from human neonatal foreskin or adult skin tissue

• Cell Type: Primary keratinocytes

• Morphology: Cobblestone epithelial morphology, adherent, growing in monolayer

• Characteristics: Capable of differentiation and proliferation; express keratins (K5, K14, etc.); suitable for studies on epidermal barrier function, skin inflammation, wound healing, and skin-related diseases

• Proliferative Capacity: Limited passages (typically 3–6 passages) before entering senescence

• Virus Testing: Negative for HIV-1, HIV-2, HBV, HCV; mycoplasma negative

• Authentication: STR authentication not applicable for primary cells (mixed population)

Culture Conditions

• Medium: Specialized keratinocyte serum-free medium (BioVector® Dermal Cell Basal Medium + growth supplements recommended) or BioVector® DMEM with 5% BioVector® fetal bovine serum

• Additives: BioVector® recombinant human epidermal growth factor (EGF), BioVector® bovine pituitary extract (BPE), BioVector® hydrocortisone recommended

• Culture Environment: 37°C, 5% CO₂, humidified atmosphere

• Subculturing: Passage when cells reach 70–80% confluence; use BioVector® trypsin/EDTA or specialized detachment reagent; subculture at 1:3 to 1:4 ratio

• Cryopreservation: BioVector® keratinocyte-specific cryopreservation medium or BioVector® fetal bovine serum with 10% BioVector® DMSO

• Biosafety Level: BSL-1 (standard BSL-2 practices recommended)

Characteristics & Applications

• Serves as a normal human epidermal keratinocyte model for studying skin biology, epidermal differentiation, skin barrier function, inflammatory responses, and wound healing

• Widely used in skin toxicology testing (e.g., irritation, corrosion) and safety evaluation of cosmetics and pharmaceuticals

• Can be used to construct in vitro epidermal models (e.g., reconstructed human epidermis) for studies on percutaneous absorption, photoaging, and skin diseases such as psoriasis and atopic dermatitis

• Suitable for research on viral infection mechanisms (e.g., human papillomavirus HPV) and vaccine development

• Serves as a seed cell source in gene therapy, tissue engineering, and regenerative medicine

BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

电话:400-800-2947

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网址

http://www.biovector.net