M4A1-M2B9 BioVector®小鼠抗人 CD20 单克隆抗体杂交瘤细胞株Mouse Anti-Human CD20 Monoclonal Antibody Hybridoma Cell Line

小鼠抗人 CD20 单克隆抗体杂交瘤细胞系 M4A1-M2B9 / Mouse Anti-Human CD20 Monoclonal Antibody Hybridoma Cell Line M4A1-M2B9

数据表 / Datasheet

细胞系信息

• 名称：BioVector® M4A1-M2B9 细胞系

• 详细名称：小鼠抗人 CD20 单克隆抗体杂交瘤细胞系 M4A1-M2B9

• 来源：由小鼠 B 细胞与骨髓瘤细胞融合建立的杂交瘤细胞株，分泌抗人 CD20 单克隆抗体

• 免疫原：人 CD20 抗原

• 抗体亚型：IgG2a（κ轻链）

• 形态：悬浮或半贴壁生长，圆形或短梭形，多呈集落状

• 主要特征：稳定分泌高亲和力抗人 CD20 单克隆抗体，广泛用于 B 细胞淋巴瘤研究和靶向治疗开发

培养条件

• 培养基：BioVector® RPMI 1640 培养基，添加 10–20% BioVector® 胎牛血清

• 添加剂：建议添加 2 mM BioVector® L-谷氨酰胺、1 mM BioVector® 丙酮酸钠

• 培养环境：37°C，5% CO₂，饱和湿度

• 传代方法：每 2–3 天按 1:3 至 1:5 比例传代；维持细胞密度在 2–5 × 10⁵ 个细胞/mL

• 冻存液：BioVector® 胎牛血清含 5–10% BioVector® DMSO

• 生物安全等级：BSL-1（建议按 BSL-2 操作）

特征与用途

• 持续分泌抗人 CD20 单克隆抗体，可用于抗体纯化、功能研究及诊断试剂开发

• 作为杂交瘤细胞模型，用于单克隆抗体生产优化、基因工程改造及稳定性研究

• 在 B 细胞相关疾病（如淋巴瘤、自身免疫病）的靶向治疗研究中具有重要应用价值

Cell Line Information

• Name: BioVector® M4A1-M2B9 Cell Line

• Detailed Name: Mouse Anti-Human CD20 Monoclonal Antibody Hybridoma Cell Line M4A1-M2B9

• Source: Hybridoma cell line established by fusion of mouse B cells with myeloma cells; secretes anti-human CD20 monoclonal antibody

• Immunogen: Human CD20 antigen

• Antibody Isotype: IgG2a (κ light chain)

• Morphology: Suspension or semi-adherent growth, round or short spindle-shaped, often in clusters

• Key Features: Stably secretes high-affinity anti-human CD20 monoclonal antibody; widely used in B-cell lymphoma research and targeted therapy development

Culture Conditions

• Medium: BioVector® RPMI 1640 supplemented with 10–20% BioVector® fetal bovine serum

• Additives: 2 mM BioVector® L-glutamine, 1 mM BioVector® sodium pyruvate recommended

• Culture Environment: 37°C, 5% CO₂, humidified atmosphere

• Subculturing: Split at 1:3 to 1:5 ratio every 2–3 days; maintain cell density between 2–5 × 10⁵ cells/mL

• Cryopreservation: BioVector® fetal bovine serum with 5–10% BioVector® DMSO

• Biosafety Level: BSL-1 (standard BSL-2 practices recommended)

Characteristics & Applications

• Continuously secretes anti-human CD20 monoclonal antibody; suitable for antibody purification, functional studies, and diagnostic reagent development

• Serves as a hybridoma model for monoclonal antibody production optimization, genetic engineering, and stability studies

• Valuable for targeted therapy research in B-cell-associated diseases such as lymphoma and autoimmune disorders

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