2B4-T BioVector®人前列腺癌细胞系 / Human Prostate Cancer Cell Line BioVector NTCC典型培养物保藏中心

BioVector® 2B4-T 人前列腺癌细胞系 / Human Prostate Cancer Cell Line 数据说明书

产品名称: BioVector® 2B4-T 细胞
同义词: BioVector® 2B4 T; BioVector® 2B4
中文名称: BioVector® 2B4-T 人前列腺癌细胞系
英文名称: BioVector® 2B4-T Human Prostate Cancer Cell Line
产品类别: 肿瘤细胞系
物种来源: 人 (Homo sapiens)
组织来源: 前列腺
疾病: 前列腺癌
形态特性: 上皮细胞样
生长特性: 贴壁生长
构建方法: 从人前列腺癌组织分离建立 -2-3-4
注意事项: 细胞系名称“2B4”与在免疫细胞（如NK细胞、T细胞）上表达的2B4受体（CD244）蛋白不同。此细胞系为肿瘤细胞，不表达该免疫受体 -6-8-9。
培养基: BioVector® 2B4-T 细胞专用完全培养基（基础成分为DMEM培养基，添加10%胎牛血清和1%青霉素-链霉素） -2-3-4
培养条件: 气相：95%空气，5%二氧化碳；温度：37°C -2-3-4
传代方法: 当细胞融合度达到80-90%时，推荐使用 BioVector® 专用传代试剂（如0.25%胰蛋白酶-EDTA）进行消化，按1:2至1:6比例进行传代，每周传代2次 -2-3-4。
冻存液: BioVector® 细胞冻存液（推荐配方：90%完全培养基 + 10% DMSO） -2-3-4
质量控制: 支原体检测：阴性 -2-3-4；无菌检测：阴性
储存与运输: 液氮或干冰运输与保存
生物安全等级: 1
仅供科研使用

English:
BioVector® 2B4-T Human Prostate Cancer Cell Line Datasheet

Product Name: BioVector® 2B4-T Cells
Synonyms: BioVector® 2B4 T; BioVector® 2B4
Chinese Name: BioVector® 2B4-T 人前列腺癌细胞系
English Name: BioVector® 2B4-T Human Prostate Cancer Cell Line
Product Category: Cancer Cell Line
Species of Origin: Human (Homo sapiens)
Tissue of Origin: Prostate
Disease: Prostate Cancer
Morphology: Epithelial-like
Growth Characteristics: Adherent
Derivation: Established from human prostate cancer tissue -2-3-4
Important Note: The cell line name "2B4" is distinct from the 2B4 receptor protein (CD244) expressed on immune cells such as NK cells and T cells. This cell line is a tumor cell line and does not express that immune receptor -6-8-9.
Culture Medium: BioVector® Complete Medium for 2B4-T Cells (Composition: DMEM medium supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin) -2-3-4
Culture Conditions: Atmosphere: 95% air, 5% CO₂; Temperature: 37°C -2-3-4
Subculturing Method: When cells reach 80-90% confluence, recommended to use BioVector® Subculture Reagent (e.g., 0.25% Trypsin-EDTA). Subculture at a split ratio of 1:2 to 1:6, twice per week -2-3-4.
Freeze Medium: BioVector® Cell Freeze Medium (Recommended composition: 90% complete medium + 10% DMSO) -2-3-4
Quality Control: Mycoplasma: Negative -2-3-4; Sterility: Negative
Storage and Shipment: Stored and shipped in liquid nitrogen or on dry ice
Biosafety Level: 1
For Research Use Only

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