Streptomyces sp. NRRL 5331 BioVector®链霉菌菌株 / Streptomyces sp. NRRL 5331 Strain

BioVector® Streptomyces sp. NRRL 5331 链霉菌菌株 / Streptomyces sp. NRRL 5331 Strain 数据说明书

产品名称: BioVector® Streptomyces sp. NRRL 5331 菌种
中文名称: BioVector® NRRL 5331 链霉菌菌株
英文名称: BioVector® Streptomyces sp. NRRL 5331 Strain
产品类别: 放线菌
菌株类型: 链霉菌属 (Streptomyces)
保藏编号: NRRL 5331 (Agricultural Research Service Culture Collection)
同义词: Streptomyces sp. X-11085
分离来源: 土壤样品
主要用途: 生产氨基乙氧基乙烯甘氨酸；植物生长调节剂研究；乙烯生物合成抑制机制研究；氨基酸代谢途径研究

代谢产物:

1. 氨基乙氧基乙烯甘氨酸 (Aminoethoxyvinylglycine, AVG): 乙烯合成酶抑制剂，可抑制植物乙烯生成，延缓果实成熟、延长保鲜期，防止落花落果 -2
   基因研究:

2. threonine biosynthesis gene cluster: 已克隆并测序了参与苏氨酸生物合成的hom, thrB和thrC基因，它们分别编码高丝氨酸脱氢酶、高丝氨酸激酶和苏氨酸合酶。这些基因的排列顺序为hom-thrC-thrB，与天蓝色链霉菌相似 -1-9

3. ask-asd operon: 已克隆并测序了苏氨酸途径的前两个基因ask和asd，它们编码天冬氨酸激酶和天冬氨酸半醛脱氢酶，以双顺反子操纵子形式组织。asd基因的破坏会显著降低AVG产量，表明该操纵子参与AVG生物合成 -5-8

4. AVG生物合成途径: hom基因的破坏完全阻断AVG生物合成，表明高丝氨酸是通向AVG的天冬氨酸衍生生物合成途径的分支点，AVG的乙烯基甘氨酸部分的四个碳原子来源于高丝氨酸 -7-10
   培养基: BioVector® ISP-2 培养基（酵母提取物-麦芽提取物琼脂）；BioVector® 胰蛋白酶大豆肉汤
   培养基配方:

• ISP-2: 酵母提取物 4.0g，麦芽提取物 10.0g，葡萄糖 4.0g，琼脂 20.0g，蒸馏水 1.0L，pH 7.3

• 种子培养基: 胰蛋白酶大豆肉汤
  培养温度: 28°C
  保存方法: 4°C短期保存（斜面）；-80°C甘油保存或液氮长期保存
  冻存保护剂: 20% 甘油 或 10% DMSO
  产品形式: 冻干粉 / 斜面培养物 / 甘油菌
  生物安全等级: 1
  质量控制: 无菌检测：阴性；活力检测：复苏后生长良好；代谢产物检测：阳性
  储存与运输: 4-10°C冷藏运输（短期），干冰或液氮运输（长期）
  仅供科研使用

English:
BioVector® Streptomyces sp. NRRL 5331 Strain Datasheet

Product Name: BioVector® Streptomyces sp. NRRL 5331 Strain
Chinese Name: BioVector® NRRL 5331 链霉菌菌株
English Name: BioVector® Streptomyces sp. NRRL 5331 Strain
Product Category: Actinobacteria
Strain Type: Streptomyces species
Deposit Number: NRRL 5331 (Agricultural Research Service Culture Collection)
Synonyms: Streptomyces sp. X-11085
Isolation Source: Soil sample
Primary Applications: Production of aminoethoxyvinylglycine; plant growth regulator research; ethylene biosynthesis inhibition mechanism studies; amino acid metabolic pathway research
Metabolites:

1. Aminoethoxyvinylglycine (AVG): Ethylene synthesis inhibitor; inhibits ethylene production in plants, delays fruit ripening, extends shelf life, and prevents flower and fruit abscission -2
   Genetic Studies:

2. Threonine Biosynthesis Gene Cluster: The hom, thrB, and thrC genes involved in threonine biosynthesis have been cloned and sequenced, encoding homoserine dehydrogenase, homoserine kinase, and threonine synthase, respectively. These genes are organized in the order hom-thrC-thrB, similar to Streptomyces coelicolor -1-9

3. ask-asd Operon: The first two genes of the threonine pathway, ask and asd, encoding aspartokinase and aspartate semialdehyde dehydrogenase, have been cloned and sequenced. They are organized as a bicistronic operon. Disruption of asd significantly decreases AVG production, supporting the involvement of this operon in AVG biosynthesis -5-8

4. AVG Biosynthetic Pathway: Disruption of the hom gene completely blocks AVG biosynthesis, indicating that homoserine is the branching point of the aspartic acid-derived biosynthetic route leading to AVG. The four carbon atoms of the vinylglycine moiety of AVG derive from homoserine -7-10
   Culture Medium: BioVector® ISP-2 Medium (Yeast Extract-Malt Extract Agar); BioVector® Trypticase Soy Broth
   Medium Composition:

• ISP-2: Yeast extract 4.0g, malt extract 10.0g, glucose 4.0g, agar 20.0g, distilled water 1.0L, pH 7.3

• Seed Medium: Trypticase soy broth
  Culture Temperature: 28°C
  Storage Methods: Short-term storage at 4°C (slants); long-term storage at -80°C in glycerol or in liquid nitrogen
  Cryoprotectant: 20% glycerol or 10% DMSO
  Product Format: Freeze-dried powder / Slant culture / Glycerol stock
  Biosafety Level: 1
  Quality Control: Sterility: Negative; Viability: Good growth upon revival; Metabolite Production: Positive
  Storage and Shipment: Refrigerated transport at 4-10°C (short-term), dry ice or liquid nitrogen transport (long-term)
  For Research Use Only

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