BioVector® Streptomyces sp. X-11085 链霉菌菌株 / Streptomyces sp. X-11085 Strain

BioVector® Streptomyces sp. X-11085 链霉菌菌株 / Streptomyces sp. X-11085 Strain 数据说明书

产品名称: BioVector® Streptomyces sp. X-11085 菌种
中文名称: BioVector® X-11085 链霉菌菌株
英文名称: BioVector® Streptomyces sp. X-11085 Strain
产品类别: 放线菌
菌株类型: 链霉菌属 (Streptomyces)
保藏编号: NRRL 5331 -1-3
分离来源: 土壤样品，采集自美国加利福尼亚州阿林顿 -1-3
主要用途: 生产抗生素L-反式-2-氨基-4-(2-氨基乙氧基)-3-丁烯酸；生产脱落剂L-2-氨基-4-(2-氨基乙氧基)-丁酸；生产5-羟基-L-赖氨酸 -1-2-3

代谢产物:

1. L-反式-2-氨基-4-(2-氨基乙氧基)-3-丁烯酸 (L-trans-2-amino-4-(2-aminoethoxy)-3-butenoic acid): 具有抗菌和驱虫活性 -1

2. L-2-氨基-4-(2-氨基乙氧基)-丁酸 (L-2-amino-4-(2-aminoethoxy)-butanoic acid, Aviglycine): 可增强果实乙烯产生，用作脱落剂 -2-3-4

3. 5-羟基-L-赖氨酸 (5-Hydroxy-L-lysine): 羟基化氨基酸 -5
   形态特征:

4. 气生菌丝: 丰富，粉末状，孢子丝呈直或弯曲状，无螺旋 -1

5. 孢子: 长圆柱形，表面光滑至略皱，有鞘膜，大小0.6×0.4至1.0×0.5微米 -1

6. 孢子颜色: 大量孢子呈灰白色、象牙色至淡黄色 -1

7. 基内菌丝: 黄色至橙黄色至浅棕色 -1
   培养特征:

• 最适生长温度: 28°C -1-3

• 生长温度范围: 28-35°C生长良好，45°C以上不生长 -1

• 最适pH: 未明确报道，通常中性偏碱

• 需氧类型: 好氧 -1-3
  生理生化特性:

• 黑色素产生: 在蛋白胨-铁培养基上高度产黑色素 -1

• 明胶液化: 阳性 -1

• 淀粉水解: 阳性 -1

• 牛奶: 生长良好，中等胨化 -1
  培养基: BioVector® ISP-2 培养基（酵母提取物-麦芽提取物琼脂）；BioVector® 胰蛋白酶大豆肉汤（种子培养基）-1-3
  培养基配方:

• ISP-2: 酵母提取物 4.0g，麦芽提取物 10.0g，葡萄糖 4.0g，琼脂 20.0g，蒸馏水 1.0L，pH 7.3

• 种子培养基: 胰蛋白酶大豆肉汤（含胰酶消化酪蛋白 15g/L，大豆蛋白胨 5g/L，葡萄糖 2.5g/L，磷酸氢二钾 2.5g/L，氯化钠 5g/L）-3

• 发酵培养基: 葡萄糖 10.0g，蛋白胨 5.0g，酵母提取物 3.0g，Fe(NH4)2(SO4)2·6H2O 0.031g，蒸馏水 1.0L -3
  培养温度: 28°C -1-3
  保存方法: 4°C短期保存（斜面）；-80°C甘油保存或液氮长期保存
  冻存保护剂: 20% 甘油 或 10% DMSO
  产品形式: 冻干粉 / 斜面培养物 / 甘油菌
  生物安全等级: 1
  质量控制: 无菌检测：阴性；活力检测：复苏后生长良好；形态学鉴定：符合标准描述；代谢产物检测：阳性
  储存与运输: 4-10°C冷藏运输（短期），干冰或液氮运输（长期）
  仅供科研使用

English:
BioVector® Streptomyces sp. X-11085 Strain Datasheet

Product Name: BioVector® Streptomyces sp. X-11085 Strain
Chinese Name: BioVector® X-11085 链霉菌菌株
English Name: BioVector® Streptomyces sp. X-11085 Strain
Product Category: Actinobacteria
Strain Type: Streptomyces species
Deposit Number: NRRL 5331 -1-3
Isolation Source: Soil sample collected from Arlington, California, USA -1-3
Primary Applications: Production of antibiotic L-trans-2-amino-4-(2-aminoethoxy)-3-butenoic acid; production of abscission agent L-2-amino-4-(2-aminoethoxy)-butanoic acid; production of 5-hydroxy-L-lysine -1-2-3
Metabolites:

1. L-trans-2-amino-4-(2-aminoethoxy)-3-butenoic acid: Exhibits antimicrobial and anthelmintic activity -1

2. L-2-amino-4-(2-aminoethoxy)-butanoic acid (Aviglycine): Enhances ethylene production in fruits, used as an abscission agent -2-3-4

3. 5-Hydroxy-L-lysine: Hydroxylated amino acid -5
   Morphological Characteristics:

4. Aerial Mycelium: Abundant, powdery; sporophores straight to flexuous, no spirals -1

5. Spores: Long cylindrical, smooth to slightly rugose with a sheath, measuring 0.6×0.4 to 1.0×0.5 μm -1

6. Spore Mass Color: Sandy-buff, ivory to light buff -1

7. Substrate Mycelium: Yellow to orangy yellow to light brown -1
   Culture Characteristics:

• Optimal Growth Temperature: 28°C -1-3

• Temperature Range: Grows well at 28-35°C, no growth above 45°C -1

• Optimal pH: Not specifically reported, typically neutral to slightly alkaline

• Oxygen Requirement: Aerobic -1-3
  Physiological and Biochemical Characteristics:

• Melanin Production: Highly chromogenic on peptone-iron medium -1

• Gelatin Liquefaction: Positive -1

• Starch Hydrolysis: Positive -1

• Milk: Good growth, moderate peptonization -1
  Culture Medium: BioVector® ISP-2 Medium (Yeast Extract-Malt Extract Agar); BioVector® Trypticase Soy Broth (seed medium) -1-3
  Medium Composition:

• ISP-2: Yeast extract 4.0g, malt extract 10.0g, glucose 4.0g, agar 20.0g, distilled water 1.0L, pH 7.3

• Seed Medium: Trypticase soy broth (pancreatic digest of casein 15g/L, enzymatic digest of soybean protein 5g/L, glucose 2.5g/L, dipotassium phosphate 2.5g/L, sodium chloride 5g/L) -3

• Fermentation Medium: Glucose 10.0g, peptone 5.0g, yeast extract 3.0g, Fe(NH4)2(SO4)2·6H2O 0.031g, distilled water 1.0L -3
  Culture Temperature: 28°C -1-3
  Storage Methods: Short-term storage at 4°C (slants); long-term storage at -80°C in glycerol or in liquid nitrogen
  Cryoprotectant: 20% glycerol or 10% DMSO
  Product Format: Freeze-dried powder / Slant culture / Glycerol stock
  Biosafety Level: 1
  Quality Control: Sterility: Negative; Viability: Good growth upon revival; Morphological Identification: Conforms to standard description; Metabolite Production: Positive
  Storage and Shipment: Refrigerated transport at 4-10°C (short-term), dry ice or liquid nitrogen transport (long-term)
  For Research Use Only

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