Peronophythora litchii BioVector®荔枝霜疫霉菌 / Litchi Downy Blight Fungus -BioVector NTCC典型培养物保藏中心

BioVector® Peronophythora litchii 荔枝霜疫霉菌 / Litchi Downy Blight Fungus 数据说明书

产品名称: BioVector® Peronophythora litchii 菌种
中文名称: BioVector® 荔枝霜疫霉菌
英文名称: BioVector® Peronophythora litchii (Litchi Downy Blight Fungus)
产品类别: 植物病原真菌
菌种类型: 卵菌 (Oomycete)
分类地位: 鞭毛菌亚门，卵菌纲，霜霉目，霜疫霉科 (Peronophythoraceae) -4
拉丁学名: Peronophythora litchii Chen ex Ko et al. -1-10
同义词: 荔枝霜霉病菌、荔枝疫病菌 -1
分离来源: 分离自荔枝果实/果皮 -3-10
主要用途: 植物病害研究；荔枝霜疫霉病病原菌鉴定；杀菌剂筛选；植物-病原互作研究 -3-10
形态特征:

1. 菌落形态: 在PDA培养基上生长，菌落白色至奶油色

2. -9

3. 无性阶段: 孢囊梗呈树枝状不对称分枝，无色，多隔，小梗末端尖，其上着生一个孢子囊；孢子囊无色至淡褐色，柠檬形或长椭圆形，顶端具乳头状突起 -1-4

4. 有性阶段: 产生卵孢子，球形，无色至淡黄色，表面光滑 -1-4
   生物学特性:

• 生长温度范围：11-33℃，最适生长温度：22-25℃ -4

• 孢子囊形成最适温度：22-25℃ -4

• 孢子囊萌发：8-22℃形成游动孢子，26-30℃直接萌发成芽管 -4

• 需氧类型：好氧 -3

• 对湿度要求高，高湿条件下致病性强 -4
  寄主范围: 主要侵染荔枝，亦可侵染番木瓜；人工接种可侵染番茄和丝瓜果实 -4
  培养基: BioVector® PDA 培养基（马铃薯葡萄糖琼脂培养基）-3-8
  培养基配方: 马铃薯提取液 1.0L，葡萄糖 20.0g，琼脂 15.0g，pH自然 -3
  培养温度: 25-28°C -3-8
  保存方法: 4-10°C冷藏保存（短期）；液氮超低温冷冻保存（长期）-3-5
  冻存保护剂: 10%甘油 或 5-15% DMSO -5
  冻存方法: 缓慢降温（1°C/min至-40°C）后转入液氮保存 -5
  复苏方法: 38°C水浴快速解冻 -5
  产品形式: 冻干粉 / 斜面培养物 / 甘油菌 -3-8
  生物安全等级: 1 -10
  质量控制: 无菌检测：阴性；活力检测：复苏后生长良好；形态学鉴定：符合标准描述
  储存与运输: 4-10°C冷藏运输（短期），干冰或液氮运输（长期）
  仅供科研使用

English:
BioVector® Peronophythora litchii (Litchi Downy Blight Fungus) Datasheet

Product Name: BioVector® Peronophythora litchii Strain
Chinese Name: BioVector® 荔枝霜疫霉菌
English Name: BioVector® Peronophythora litchii (Litchi Downy Blight Fungus)
Product Category: Plant Pathogenic Fungus
Strain Type: Oomycete
Taxonomic Classification: Mastigomycotina, Oomycetes, Peronosporales, Peronophythoraceae -4
Scientific Name: Peronophythora litchii Chen ex Ko et al. -1-10
Synonyms: Litchi downy blight fungus -1
Isolation Source: Isolated from litchi fruit/pericarp -3-10
Primary Applications: Plant pathology research; Identification of litchi downy blight pathogen; Fungicide screening; Plant-pathogen interaction studies -3-10
Morphological Characteristics:

1. Colony Morphology: Whitish cream colonies on PDA medium -9

2. Asexual Stage: Sporangiophores are tree-like, asymmetrically branched, colorless, with multiple septa; sporangia are colorless to light brown, lemon-shaped or long ellipsoid, with apical papillae -1-4

3. Sexual Stage: Produces oospores, spherical, colorless to light yellow, smooth-walled -1-4
   Biological Characteristics:

• Temperature Range for Growth: 11-33°C, Optimal Growth Temperature: 22-25°C -4

• Optimal Temperature for Sporangia Formation: 22-25°C -4

• Sporangia Germination: 8-22°C (zoospore formation), 26-30°C (direct germination into germ tubes) -4

• Oxygen Requirement: Aerobic -3

• Requires high humidity for infection -4
  Host Range: Mainly infects litchi; can also infect papaya; under artificial inoculation, can infect tomato and loofah fruits -4
  Culture Medium: BioVector® PDA Medium (Potato Dextrose Agar) -3-8
  Medium Composition: Potato extract 1.0L, glucose 20.0g, agar 15.0g, natural pH -3
  Culture Temperature: 25-28°C -3-8
  Storage Methods: 4-10°C refrigeration (short-term); Liquid nitrogen ultra-low temperature freezing (long-term) -3-5
  Cryoprotectant: 10% glycerol or 5-15% DMSO -5
  Freezing Method: Slow cooling (1°C/min to -40°C) followed by transfer to liquid nitrogen -5
  Thawing Method: Rapid thawing in 38°C water bath -5
  Product Format: Freeze-dried powder / Slant culture / Glycerol stock -3-8
  Biosafety Level: 1 -10
  Quality Control: Sterility Test: Negative; Viability Test: Good growth upon revival; Morphological Identification: Conforms to standard description
  Storage and Shipment: Refrigerated transport at 4-10°C (short-term), dry ice or liquid nitrogen transport (long-term)
  For Research Use Only

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