VCSM13 BioVector®干扰抗性辅助噬菌体 / VCSM13 Interference-Resistant Helper Phage

BioVector® VCSM13干扰抗性辅助噬菌体 / VCSM13 Interference-Resistant Helper Phage 数据说明书

产品名称: BioVector® VCSM13 辅助噬菌体
中文名称: BioVector® VCSM13 干扰抗性辅助噬菌体
英文名称: BioVector® VCSM13 Interference-Resistant Helper Phage
产品类别: 辅助噬菌体
噬菌体类型: 丝状噬菌体
衍生来源: 由R408辅助噬菌体衍生而来
基因组大小: 约4 Kb
抗性标记: 卡那霉素抗性
滴度: >1 × 10¹¹ pfu/ml
储存缓冲液: 含7% DMSO的保护液
用途与应用:

1. 噬菌体展示: 为噬菌粒载体（如pComb3、pComb3XSS）提供复制和包装必需的所有基因产物，使噬菌粒能够包装成完整的噬菌体颗粒

2. 单链DNA制备: 用于从已切除的噬菌粒中产生单链DNA

3. 文库扩增: 用于噬菌体展示文库的再扩增和生物淘选过程中的辅助
   作用机制: VCSM13辅助噬菌体为缺失了包装必需基因的噬菌粒提供反式互补，使携带外源片段的噬菌粒得以复制和组装
   推荐宿主菌: 大肠杆菌 XL1-Blue MRF' 菌株（因其含有supE44突变，提供谷氨酰胺抑制tRNA）
   感染条件: 推荐感染复数（MOI）为20:1（噬菌体:细胞比）
   质控载体: BioVector® pComb3 噬菌粒载体（用于功能验证）
   宿主菌: BioVector® XL1-Blue MRF' 感受态细胞
   质量控制: 滴度检测：≥1 × 10¹¹ pfu/ml；功能验证：可有效拯救噬菌粒；无菌检测：阴性
   储存与运输: 4°C短期储存，-80°C或干冰长期运输与保存
   仅供科研使用

BioVector® VCSM13 Helper Phage Datasheet

Product Name: BioVector® VCSM13 Helper Phage
Chinese Name: BioVector® VCSM13 干扰抗性辅助噬菌体
English Name: BioVector® VCSM13 Interference-Resistant Helper Phage
Product Category: Helper Phage
Phage Type: Filamentous Phage
Derived From: Derived from R408 helper phage
Genome Size: Approximately 4 Kb
Selection Marker: Kanamycin Resistance
Titer: >1 × 10¹¹ pfu/ml
Storage Buffer: Protective buffer containing 7% DMSO
Applications:

1. Phage Display: Provides all essential gene products required for replication and packaging of phagemid vectors (e.g., pComb3, pComb3XSS), enabling the packaging of phagemids into complete phage particles

2. Single-Stranded DNA Preparation: Used to generate single-stranded DNA from excised phagemids

3. Library Amplification: Used for reamplification of phage display libraries and during bio-panning procedures
   Mechanism of Action: VCSM13 helper phage provides trans-complementation of packaging-essential genes missing in phagemids, allowing replication and assembly of phagemids carrying foreign inserts
   Recommended Host Strain: E. coli XL1-Blue MRF' strain (contains supE44 mutation providing glutamine suppressor tRNA)
   Infection Conditions: Recommended multiplicity of infection (MOI) of 20:1 (phage-to-cell ratio)
   Control Vector: BioVector® pComb3 Phagemid Vector (for functional validation)
   Host Strain: BioVector® XL1-Blue MRF' Competent Cells
   Quality Control: Titer Validation: ≥1 × 10¹¹ pfu/ml; Functional Validation: Efficient phagemid rescue; Sterility: Negative
   Storage and Shipment: Store at 4°C for short-term; store at -80°C or ship on dry ice for long-term storage
   For Research Use Only

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