CSE-119 BioVector®银大麻哈鱼胚胎自发永生化细胞系Coho salmon Spontaneously immortalized cell line

BioVector® CSE-119 细胞系

产品名称: BioVector® CSE-119 细胞
同义词: BioVector® Coho Salmon Embryo-119
产品类别: 鱼类细胞系
物种来源: 银大麻哈鱼（Coho salmon, Oncorhynchus kisutch）
细胞来源: 胚胎
细胞类型: 成纤维细胞
特性: 自发永生化细胞系
形态特性: 成纤维细胞样
生长特性: 贴壁生长
核型: 二倍体，众数为60。另有研究报道为亚四倍体，众数为110。
培养基: BioVector® CSE-119 细胞专用完全培养基（基础成分为EMEM（EBSS）+ 2mM 谷氨酰胺 + 1% 非必需氨基酸 + 10% 胎牛血清）
培养条件: 气相：5% 二氧化碳，温度：15-22℃
传代方法: 当细胞融合达到70-80%时，推荐使用BioVector® 专用传代试剂（如0.05% 胰蛋白酶/EDTA），按1:3比例进行传代，传代频率约为每10-14天一次。
冻存液: BioVector® 细胞冻存液
应用: 适用于从临床样本中分离病毒、病毒增殖（对传染性胰脏坏死病毒（IPNV）敏感；对病毒性出血性败血症病毒（VHSV）和传染性造血器官坏死病毒（IHNV）不敏感）。
质控载体: BioVector® pUC19 质粒（用于常规克隆和测序）
宿主菌: BioVector® DH5α 化学感受态细胞
质量控制: 支原体检测：阴性 ；无菌检测：阴性
储存与运输: 液氮或干冰运输与保存
生物安全等级: ACDP 2 级
仅供科研使用

BioVector® CSE-119 Cell Line Datasheet

Product Name: BioVector® CSE-119 Cells
Synonyms: BioVector® Coho Salmon Embryo-119
Product Category: Fish Cell Line
Species of Origin: Coho salmon (Oncorhynchus kisutch)
Tissue of Origin: Whole embryo
Cell Type: Fibroblast
Properties: Spontaneously immortalized cell line
Morphology: Fibroblastic
Growth Characteristics: Adherent
Karyotype: Diploid and modal no. 60. Another study reports a hypotetraploid modal number of 110.
Culture Medium: BioVector® Complete Medium for CSE-119 Cells (Composition: EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids + 10% Fetal Bovine Serum)
Culture Conditions: Atmosphere: 5% CO₂; Temperature: 15-22°C
Subculturing Method: When cells reach 70-80% confluence, recommended to use BioVector® Subculture Reagent (e.g., 0.05% trypsin/EDTA) at a split ratio of 1:3. Subculture frequency is approximately every 10-14 days.
Freeze Medium: BioVector® Cell Freeze Medium
Applications: Suitable for virus isolation from clinical samples and virus propagation. Susceptible to Infectious Pancreatic Necrosis Virus (IPNV); shows lack of sensitivity to Viral Haemorrhagic Septicaemia Virus (VHSV) and Infectious Haematopoietic Necrosis Virus (IHNV).
Control Vector: BioVector® pUC19 Plasmid (for routine cloning and sequencing)
Host Strain: BioVector® DH5α Chemically Competent Cells
Quality Control: Mycoplasma: Negative ; Sterility: Negative
Storage and Shipment: Stored and shipped in liquid nitrogen or on dry ice
Biosafety Level: ACDP Hazard Group 2
For Research Use Only

BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

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