RPTEC/TERT1 BioVector®人肾近端肾小管上皮永生化细胞株human Renal Proximal Tubule Epithelial Cell Line
- 价 格：¥49960
- 货 号：BioVector®-RPTEC/TERT1
- 产 地：北京
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作微信:1843439339 (QQ同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
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RPTEC/TERT1 Cell Line细胞系
Product Name / 产品名称:
BioVector® RPTEC/TERT1 Cell Line
BioVector® RPTEC/TERT1细胞系
Description / 描述:
The BioVector® RPTEC/TERT1 (human Renal Proximal Tubule Epithelial/Telomerase Reverse Transcriptase 1) cell line is a well-characterized, telomerase-immortalized human cell line derived from the proximal tubule of a healthy adult male kidney -4-10. It was established by transfecting primary renal proximal tubule epithelial cells with the human telomerase reverse transcriptase (hTERT) gene, which allows the cells to bypass replicative senescence while retaining the key morphological and functional characteristics of their primary cell counterparts -10. This results in a stable, reproducible, and physiologically relevant in vitro model of the human renal proximal tubule -5-7.
BioVector® RPTEC/TERT1（人肾近端肾小管上皮/端粒酶逆转录酶1） 细胞系是一种特性明确、经端粒酶永生化的人源细胞系，来源于健康成年男性供体的肾近端小管 -4-10。它通过将人端粒酶逆转录酶基因转入原代肾近端小管上皮细胞而建立，使细胞能够绕过复制性衰老，同时保留其原代细胞的关键形态和功能特征 -10。这使其成为一个稳定、可重复且生理相关性高的人肾近端小管体外模型 -5-7。
Species / 物种:
Human / 人
Tissue Origin / 组织来源:
Kidney; Proximal Tubule (Cortex) / 肾脏；近端肾小管（皮质）
Morphology / 形态:
Epithelial-like / 上皮样
Growth Characteristics / 生长特性:
Adherent; forms characteristic "domes" at high density, indicating functional cell polarization and vectorial transport -4-10.
贴壁生长；高密度时形成特征性的"穹窿"结构，表明细胞功能极化和定向转运能力 -4-10。
Key Applications / 主要应用:
Nephrotoxicity screening (drugs, environmental toxicants like cadmium) -3-5-7
Biogerontology and tissue engineering -10
Studying renal progenitor cell biology and tubular regeneration -3
生物老年学及组织工程 -10
研究肾祖细胞生物学及肾小管再生 -3
Key Features & Functional Characteristics / 关键特征与功能特性:
Primary-like Function / 类原代功能: Retains many differentiated features of primary proximal tubule cells, including response to parathyroid hormone (PTH) but not to arginine vasopressin (AVP), sodium-dependent phosphate uptake, and functional megalin/cubilin-mediated endocytosis system -4-10.
Barrier Integrity / 屏障完整性: Forms tight junctions and maintains a stable Trans-Epithelial Electrical Resistance (TEER) when cultured on permeable supports, demonstrating intact functional barrier -4-7.
Transporter Expression / 转运蛋白表达: Expresses key proximal tubule transporters and markers, such as Aminopeptidase N (CD13), γ-Glutamyl Transferase (GGT), and E-cadherin -4. Transporter expression has been shown to mature over time in culture -7.
Progenitor Population / 祖细胞亚群: Contains a subpopulation of cells co-expressing CD133 and CD24, which exhibit progenitor/stem cell-like properties, such as forming nephrospheres and differentiating into multiple lineages -3.
保留了原代近端小管细胞的许多分化特征，包括对甲状旁腺激素有反应但对精氨酸加压素无反应、钠依赖性磷酸盐摄取以及功能性巨蛋白/立方蛋白介导的内吞系统 -4-10。
表达关键的近端小管转运蛋白和标志物，如氨基肽酶N、γ-谷氨酰转移酶和E-钙黏蛋白 -4。研究表明，转运蛋白的表达会随着培养时间的延长而成熟 -7。
包含一个共表达CD133和CD24的细胞亚群，该亚群表现出祖细胞/干细胞的特性，例如形成肾球和分化为多种谱系的能力 -3。
Engineered Variants / 工程改造细胞株:
| Product Name / 产品名称 | Description / 描述 |
|---|---|
| BioVector® RPTEC/TERT1 OCT2 Cell Line | Stably expresses the Organic Cation Transporter 2 (OCT2, SLC22A2) for enhanced cationic drug transport studies -1. 稳定表达有机阳离子转运蛋白2，用于增强阳离子药物转运研究 -1。 |
| BioVector® RPTEC/TERT1 OAT1 Cell Line | Stably expresses the Organic Anion Transporter 1 (OAT1, SLC22A6) for anionic drug transport and toxicity research -6. 稳定表达有机阴离子转运蛋白1，用于阴离子药物转运和毒性研究 -6。 |
Culture Conditions / 培养条件:
| Parameter / 参数 | Specification / 规格 |
|---|---|
| Recommended Medium / 推荐培养基 | BioVector® Renal Epithelial Cell Growth Medium (DMEM:F12-based) |
| Kit Supplements / 培养基添加物 | BioVector® RPTEC Growth Kit (containing EGF, Hydrocortisone, Insulin, Transferrin, etc.) |
| Selective Antibiotic (Parental) / 选择性抗生素 (亲本株) | BioVector® G418 Sulfate Solution (0.1 mg/mL final concentration) -4 |
| Selective Antibiotic (Variants) / 选择性抗生素 (改造株) | BioVector® Puromycin Solution (e.g., 0.3 µg/mL for OAT1) or BioVector® G418 Sulfate Solution as appropriate -1-6 |
| Serum / 血清 | Not required (serum-free system) / 无需添加（无血清培养系统） |
| Subculture / 传代 | Detach with BioVector® Trypsin-EDTA for Primary Cells, neutralize with BioVector® Soybean Trypsin Inhibitor-4-6. |
| Temperature / 温度 | 37°C with 5% CO2 |
Quality Control / 质量控制:
Negative for mycoplasma and bacterial contamination. Verified for morphology, expression of epithelial markers (e.g., pan-cytokeratin, E-cadherin), and functional characteristics (e.g., dome formation) -4-6.
无支原体及细菌污染。经验证具有正常形态，表达上皮标志物（如泛角蛋白、E-钙黏蛋白），并具有功能特性（如形成穹窿）-4-6。
Storage / 存储:
BioVector® Cryopreservation Medium (with 10% DMSO) is recommended for long-term storage in liquid nitrogen vapor phase -4.
推荐使用 BioVector® 细胞冻存液（含10% DMSO）进行长期保存（液氮气相）-4。
BioVector® RPTEC Growth Kit
BioVector® RPTEC生长因子添加套装
Contains the necessary supplements (EGF, hydrocortisone, insulin, transferrin, etc.) to be added to BioVector® Renal Epithelial Cell Growth Medium for complete RPTEC/TERT1 culture.
包含必要的添加物，需添加至 BioVector® 肾上皮细胞生长培养基 中，以完成RPTEC/TERT1的培养。
BioVector® G418 Sulfate Solution
BioVector® G418硫酸盐溶液
For maintaining selective pressure on the parental RPTEC/TERT1 cell line.
用于维持亲本RPTEC/TERT1细胞系的选择性压力。
BioVector® Trypsin-EDTA for Primary Cells
BioVector® 原代细胞胰蛋白酶-EDTA消化液
Gentle enzyme solution for dissociating adherent RPTEC/TERT1 monolayers.
用于解离贴壁RPTEC/TERT1单层细胞的温和酶溶液。
BioVector® Soybean Trypsin Inhibitor
BioVector® 大豆胰蛋白酶抑制剂
Used to neutralize trypsin activity during subculture to protect cell viability.
用于传代过程中中和胰蛋白酶活性，保护细胞活力。
BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心
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