pAAV/1.2HBV BioVector®乙型肝炎病毒 (HBV) 腺相关病毒病毒质粒载体

BioVector® pAAV/1.2HBV plasmid, commonly used in research to establish chronic Hepatitis B virus (HBV) infection models in mice.

• 载体名称 (Vector Name): BioVector® pAAV/1.2HBV

• 载体类型 (Vector Type): 复制能力质粒 (Replication-competent plasmid).

• 主要组成 (Components):

  • HBV 基因组: 包含 1.2 倍长的乙型肝炎病毒 (HBV) 基因组（通常为 A 型或 D 型），该序列跨越核苷酸 1400-3182-1987.

  • AAV 骨架: 病毒序列被整合到腺相关病毒 (AAV) 2 型的反向末端重复序列 (ITR) 之间.

  • 辅助元件: 包含 WPRE (土拨鼠肝炎病毒翻译后调节元件) 和人生长激素多腺苷酸化信号 (hGH polyA).

  
  

• 用途 (Applications): 通过流体动力学注射 (Hydrodynamic Injection) 进入小鼠（如 C57BL/6）体内，用于建立长期 HBsAg 阳性的慢性 HBV 感染模型.

• 筛选标记 (Selection Marker): 氨苄青霉素 (Ampicillin) 或 卡那霉素 (Kanamycin)（具体取决于骨架变体）.

English Datasheet

• Plasmid Name: pAAV/1.2HBV

• Vector Type: AAV-based HBV expression/replication vector.

• Key Features:

  • HBV Genome: Contains a 1.2-fold over-length HBV genome (e.g., Genotype A or D) inserted to allow for autonomous viral replication and transcription.

  • Structural Elements: The HBV sequence is flanked by AAV2 Inverted Terminal Repeats (ITRs).

  • Regulatory Elements: Often includes WPRE to enhance gene expression and a human growth hormone (hGH) polyadenylation signal.

  
  

• Experimental Use: Primarily used in Hydrodynamic Injection (HI) mouse models to simulate chronic HBV infection, producing detectable serum HBsAg, HBeAg, and HBV DNA.

• Persistence: The dose of injected DNA (e.g., 6 μg vs. 20 μg) significantly impacts whether the mouse develops long-term persistence or acute clearance.

• Host Strains: Commonly used in C57BL/6 or BALB/c mice.

Supplier生产厂家:

BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心

BioVector NTCC Inc.

TEL: 400-800-2947

E-mail: biovector@163.com

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