Pt.Kich-1 BioVector®人胚胎肾脏细胞株human foetal kidney cell line

BioVector® Pt.Kich-1 cell line, a rare and specialised model for studying human foetal kidney development and associated paediatric renal pathologies.

• 细胞名称: Pt.Kich-1

• 物种: 人类 (Homo sapiens)

• 细胞类型: 胎儿肾细胞 (Foetal Kidney)

• 起源背景: 建立自一名 13.5 周龄的人类胎儿肾脏组织。

• 别名: Pt Kich 1, PtKich 1

• 生物安全等级: 1 (建议按照 BSL-2 标准处理人源样本以确保安全)

• 发育阶段: 代表人类胚胎发育的关键时期，常用于研究肾脏发生 (Nephrogenesis)。

• 表型特征: 表现为间充质 (Mesenchymal) 或未分化的肾前体细胞特征。

• 主要应用:

  • 发育生物学: 研究人类肾脏在胚胎期的发育信号通路（如 Wnt 或 Notch 通路）。

  • 病毒学: 常被用作研究某些具有嗜肾性的病毒感染机制的宿主模型。

  • 毒理学: 评估药物对发育中肾脏细胞的早期毒性影响。

  
  

• 形态学: 类成纤维细胞 (Fibroblast-like)，贴壁生长。

• 培养基: DMEM/F12 (1:1) 或 RPMI-1640 + 10% 胎牛血清 (FBS)。

• 培养条件: 37 °C, 5% CO₂。

• 传代比例: 1:2 至 1:4；当细胞达到 80-90% 融合度时使用 Trypsin-EDTA 进行消化传代。

• 冷冻保存: 90% FBS + 10% DMSO 或使用专门的无血清冻存液。

Cell Line Datasheet - English Version

• Cell Line Name: Pt.Kich-1

• Species: Human (Homo sapiens)

• Cell Type: Foetal Kidney

• Origin: Established from human foetal kidney tissue at approximately 13.5 weeks of gestation.

• Aliases: Pt Kich 1, PtKich 1

• Biosafety Level: 1 (BSL-2 handling is recommended for all human-derived cell lines).

• Developmental Stage: Represents a critical window of human nephrogenesis, providing insights into embryonic kidney maturation.

• Phenotype: Characterised by mesenchymal-like morphology and expression of renal progenitor markers.

• Research Applications:

  • Developmental Biology: Investigating the molecular cues governing the transition from mesenchymal to epithelial cells in the kidney.

  • Virology: Utilised as a substrate for studying the replication and effects of specific renal-tropic viruses.

  • Toxicity Testing: Modelling the effects of pharmaceutical compounds on developing human renal tissues.

  
  

• Morphology: Fibroblast-like; adherent monolayer.

• Culture Medium: DMEM/F12 (1:1 mixture) or RPMI-1640 supplemented with 10% FBS.

• Incubation: 37 °C with 5% CO₂.

• Subculture: Split ratio of 1:2 to 1:4 using Trypsin-EDTA; subculture when cells reach 80–90% confluency.

• Storage/Freezing: 90% FBS + 10% DMSO.

Supplier生产厂家:

BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心

BioVector NTCC Inc.

TEL: 400-800-2947

E-mail: biovector@163.com

工作微信/QQ同号: 1843439339

http://www.biovector.net