Bacillus methanolicus MGA3 BioVector®嗜甲醇芽孢杆菌-生产L-谷氨酸 (L-glutamate) 和 L-赖氨酸 (L-lysine) 的天然优良菌种

BioVector® Bacillus methanolicus MGA3, a unique thermophilic methylotroph of high industrial interest for its ability to convert methanol into valuable amino acids and bulk chemicals.

• 菌株名称: Bacillus methanolicus MGA3

• 物种: 嗜甲醇芽孢杆菌 (Bacillus methanolicus)

• 别名: BioVector® 53907

• 特征: 嗜热 (Thermophilic)、兼性甲基营养型 (Facultative Methylotroph)。

• 生物安全等级: 1 (非致病性工业菌株)。

• 甲醇利用: 能够以甲醇作为唯一的碳源和能源，通过核酮糖单磷酸 (RuMP) 途径进行代谢。

• 氨基酸生产: 该菌株是生产 L-谷氨酸 (L-glutamate) 和 L-赖氨酸 (L-lysine) 的天然优良菌种，在高温条件下具有高产潜力。

• 热稳定性: 能够在 50 °C 以上的高温下生长，显著降低了大规模发酵过程中的冷却成本并减少了杂菌污染风险。

• 代谢工程: 随着其全基因组序列的发布（约 3.3 Mb 染色体及质粒），MGA3 已成为开发“甲醇生物经济”的重要底盘生物。

• 形态学: 革兰氏阳性、产芽孢、杆状细菌。

• 培养基: 常使用 MVM 培养基（甲醇矿物盐培养基）或 SOB 培养基。

• 培养条件: 最适生长温度为 50 °C - 55 °C；pH 维持在 6.8 - 7.2。

• 遗传转化: 可通过电穿孔法转化。需要注意的是，该菌株含有内源性质粒（如 pBM19），在设计表达载体时需考虑兼容性。

Strain Datasheet - English Version

• Strain Name:Bacillus methanolicus MGA3

• Species:Bacillus methanolicus

• Aliases:BioVector®53907

• Type: Thermophilic, facultative methylotroph.

• Biosafety Level: 1

• Methanol Metabolism: Utilizes methanol as a sole carbon and energy source via the Ribulose Monophosphate (RuMP) pathway.

• Amino Acid Production: Naturally secretes high levels of L-glutamate and L-lysine, making it a key candidate for methanol-based biomanufacturing.

• Thermophily: Thrives at temperatures between 50 °C and 55 °C, offering advantages in contamination control and reduced cooling costs during industrial fermentation.

• Chassis for Synthetic Biology: Often engineered to produce non-native chemicals like cadaverine, GABA, or acetoin from methanol (Braumut et al., 2014).

• Morphology: Gram-positive, spore-forming, rod-shaped bacterium.

• Recommended Media: Methanol-supplemented Mineral Medium (MVM) or rich media like SOB for biomass. Methanol concentrations are typically kept at 200 mM to avoid toxicity.

• Incubation: Optimal growth at 50 °C with vigorous aeration.

• Genetics: Possesses a ~3.3 Mb genome and natural plasmids (pBM19 and pCH71). Genetic tools often utilize shuttle vectors capable of replicating in both B. methanolicus and E. coli.

Supplier生产厂家:

BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心

BioVector NTCC Inc.

TEL: 400-800-2947

E-mail: biovector@163.com

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http://www.biovector.net