Streptomyces albus J1074 BioVector® 白色链霉菌株-异源表达次级代谢产物如抗生素、抗肿瘤药物的常用宿主

BioVector® Streptomyces albus J1074, arguably the most popular "chassis" strain in specialized metabolism research due to its rapid growth and minimized genome.

• 菌株名称: Streptomyces albus J1074

• 物种: 白色链霉菌 (Streptomyces albus)

• 衍生背景: 衍生自 S. albus G (即 DSM 40413)。它是 SalI 限制修饰系统缺陷型变体。

• 生物安全等级: 1 (非致病性环境细菌)

• 底盘细胞 (Chassis): 它是目前异源表达（Heterologous Expression）次级代谢产物（如抗生素、抗肿瘤药物）最常用的宿主之一。

• 基因组特征: 拥有相对较小的基因组（约 6.8 Mb），且其内源性次级代谢基因簇（BGCs）较少，背景干扰低。

• 生长优势: 在链霉菌属中生长极快，在固体培养基上通常 2-3 天即可长出菌落。

• 遗传操作: 转化效率高，兼容多种接合（Conjugation）和质粒系统。

• 形态学: 典型的丝状生长，产生白色气生菌丝和灰色孢子。

• 常用培养基:

  • 液体: TSB (Tryptic Soy Broth) 或 YEME。

  • 固体: MS (Mannitol Soya) 琼脂（用于接合传代）或 R5 琼脂。

  
  

• 培养条件: 28-30 °C，液体培养需剧烈震荡（200-250 rpm）以防止菌丝结团。

• 生理特性: 能够产生多种内源性抗生素（如 Salinomycin），但在工程化研究中通常被用于生产外源产物。

Strain Datasheet - English Version

• Strain Name: BioVector® Streptomyces albus J1074

• Species:Streptomyces albus

• Origin: Derivative of S. albus G (DSM 40413). It is a defective variant of the SalI restriction-modification system.

• Biosafety Level: 1

• Heterologous Host: Widely utilized as a "workhorse" for the heterologous expression of complex natural product biosynthetic gene clusters (BGCs).

• Genome Streamlining: Possesses a streamlined genome (~6.8 Mb) compared to other streptomycetes, reducing metabolic noise and precursor competition.

• Growth Profile: Exceptionally fast growth rate for an actinomycete, typically forming colonies within 48–72 hours on agar.

• Genetic Tractability: Highly amenable to DNA uptake via intergeneric conjugation from E. coli (e.g., S17-1 or ET12567/pUZ8002).

• Morphology: Filamentous mycelial growth; produces white aerial hyphae and grey spores.

• Recommended Media:

  • Liquid: TSB or YEME for biomass and genomic DNA preparation.

  • Solid: MS (Mannitol Soya) for conjugation; R2YE or R5 for regeneration.

  
  

• Incubation:28–30 °C; liquid cultures require vigorous aeration (200–250 rpm) using baffled flasks to ensure proper oxygenation and prevent pelleting.

• Genetics: Its genome sequence is publicly available (GenBank: CP004370.1), facilitating metabolic engineering.

Supplier生产厂家:

BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心

BioVector NTCC Inc.

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E-mail: biovector@163.com

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