MC38-OVA BioVector®小鼠结肠癌稳转卵清蛋白细胞株Mouse Colon Adenocarcinoma cell line

BioVector® MC38-OVA cell line, a cornerstone model in cancer immunotherapy research used to study T-cell responses against a defined "foreign" antigen.

• 细胞名称: MC38-OVA (或 MC38-OT-I/OT-II)

• 物种: 小鼠 (Mus musculus)

• 品系背景: C57BL/6

• 细胞类型: 结肠癌 (Colon Adenocarcinoma)

• 衍生自: 亲本 MC38 细胞（来源于 C57BL/6 小鼠诱导的结肠腺癌）。

• 生物安全等级: 1 (若涉及重组病毒转导，建议在 BSL-2 下操作)

• 遗传修饰: 通过稳定转导/转染技术，使亲本 MC38 细胞持续表达卵清蛋白 (Ovalbumin, OVA)。

• 模型用途: OVA 作为一种强效的模型新抗原 (Model Neoantigen)，使其能被表达相应 TCR 的 T 细胞（如 OT-I 对应 CD8+ 或 OT-II 对应 CD4+）特异性识别。

• 应用领域:

  • 癌症疫苗研发与验证。

  • 过继性 T 细胞疗法 (ACT) 的效力评估。

  • 免疫检查点抑制剂 (ICI) 的药效评价。

  
  

• 形态学: 类上皮细胞，贴壁生长。

• 培养基: DMEM 或 RPMI 1640 + 10% FBS + 1% P/S。

• 筛选压力: 需添加 G418 (Geneticin) 或其他抗生素（取决于载体构建）以维持 OVA 的稳定表达。

• 培养条件: 37 °C, 5% CO₂。

• 倍增时间: 约 18-24 小时。

• 冷冻保存: 90% FBS + 10% DMSO。

Cell Line Datasheet - English Version

• Cell Line Name: MC38-OVA (also known as MC38-OT-I/OT-II)

• Species: Mouse (Mus musculus)

• Strain Background: C57BL/6

• Cell Type: Colon Adenocarcinoma

• Origin: Derivative of the parental MC38 line (a colon adenocarcinoma induced in C57BL/6 mice).

• Biosafety Level: 1

• Modification: Parental MC38 cells stably engineered to constitutively express the Ovalbumin (OVA) protein.

• Mechanism: OVA acts as a potent surrogate tumor-associated antigen. It allows researchers to monitor specific T-cell responses using OT-I (CD8+ specific) or OT-II (CD4+ specific) T-cells.

• Key Applications:

  • Validation of cancer vaccines.

  • Efficacy testing for Adoptive Cell Transfer (ACT).

  • In vivo syngeneic tumor models for Immunotherapy (e.g., anti-PD-1/anti-CTLA-4).

  
  

• Morphology: Epithelial-like, adherent monolayer.

• Culture Medium: DMEM or RPMI 1640 supplemented with 10% FBS and 1% Pen-Strep.

• Selection Pressure: Requires maintenance with G418 (Geneticin) or similar selection markers to ensure stable OVA expression.

• Incubation: 37 °C with 5% CO₂.

• Doubling Time: Approximately 18–24 hours.

• Storage/Freezing: 90% FBS + 10% DMSO.

Supplier生产厂家:

BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心

BioVector NTCC Inc.

TEL: 400-800-2947

E-mail: biovector@163.com

工作微信/QQ同号: 1843439339

http://www.biovector.net