首页 » HL-60_CDM BioVector®无血清培养人急性髓系白血病细胞株Human Peripheral blood; Acute Myeloid Leukemia cell line

HL-60_CDM BioVector®无血清培养人急性髓系白血病细胞株Human Peripheral blood; Acute Myeloid Leukemia cell line

  • 价  格:¥0
  • 货  号:BioVector®-HL-60_CDM
  • 产  地:北京
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BioVector®  HL-60_CDM是经典 HL-60细胞系的**无血清培养(Chemically Defined Medium)**衍生型,广泛用于研究白血病细胞的分化、增殖以及在严格控制的化学环境下的药物反应。


BioVector® HL-60_CDM 细胞系数据表
参数详细信息
细胞名称HL-60_CDM (ACC 11010)
物种来源人类 (Homo sapiens)
组织/疾病外周血;急性髓系白血病 (AML FAB M2)
细胞形态类成纤维细胞/淋巴母细胞样;圆形,悬浮生长
生长特性悬浮生长(可在无血清条件下持续增殖)
倍增时间40 小时
培养基配方无血清培养基(如 HL-60 专用 CDM 培养基)。其亲本系使用 RPMI 1640 + 10% FBS。
培养环境37°C,5% CO₂,恒温恒湿培养
传代方法维持细胞密度在 0.5–1.5 x 10⁶ 细胞/ml。每 2-3 天通过稀释或半换液传代。
标志物CD3-, CD4+, CD13(+), CD15+, CD33+, HLA-DR-, CD34-
生物安全等级1 级

HL-60 Cell Line:Acute Myeloid Leukemia Research Essentials

-α-negative myeloblastic leukemia cell line HL-60. (A) HL-60 cells were ...

English Version: HL-60_CDM Cell Line Datasheet

ParameterInformation
NameHL-60_CDM (ACC 11010)
DSMZ No.ACC 11010 (Serum-free derivative of ACC 3)
OrganismHuman (Homo sapiens)
Tissue / DiseasePeripheral blood; Acute Myeloid Leukemia (AML FAB M2)
MorphologyLymphoblast-like; round cells growing in suspension
Growth ModeSuspension
Doubling TimeApproximately 40 hours
Culture MediumChemically Defined Medium (CDM) designed for serum-free growth.
Culture Conditions37°C, 5% CO₂, humidified atmosphere
SubculturingMaintain at 0.5–1.5 x 10⁶ cells/ml. Split 1:2 to 1:5 every 2-3 days.
MarkersCD3-, CD4+, CD13(+), CD15+, CD33+, HLA-DR-, CD34-
Biosafety Level1

Key Characteristics / 关键特征
  • Serum-Free Adaptation: HL-60_CDM is specifically adapted for growth in serum-free conditions, making it an ideal model for studying the effects of specific growth factors or cytokines without interference from FBS components.

  • Differentiation Potential: Like its parent line, it can be induced to differentiate into granulocyte-like cells (via DMSO or Retinoic Acid) or macrophage-like cells (via TPA/PMA).

  • Genetic Profile: Carries the amplified MYC gene, a hallmark of the HL-60 lineage.

  • 无血清适应性: HL-60_CDM 专门适应于无血清环境,是研究特定生长因子或细胞因子作用的理想模型,排除了胎牛血清 (FBS) 成分的干扰

  • 分化潜能: 与其亲本系一样,可被诱导向粒细胞样细胞(通过 DMSO 或全反式维甲酸)或类巨噬细胞(通过 TPA/PMA)分化。

  • 遗传特征: 携带 MYC 基因扩增,这是 HL-60 谱系的典型标志。

Supplier生产厂家:

BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心

BioVector NTCC Inc.

TEL: 400-800-2947

E-mail: biovector@163.com

工作微信/QQ同号: 1843439339

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