STOSE BioVector TM自发转化的小鼠卵巢表面上皮细胞株Spontaneously Transformed Ovarian Surface Epithelial

价　　格：¥99860
货　　号：BioVector TM STOSE
产　　地：北京

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核心规格 (Core Specifications)

名称： BIoVector TM STOSE (Spontaneously Transformed Ovarian Surface Epithelial)
全称：自发转化的小鼠卵巢表面上皮细胞
物种：小鼠 (Mus musculus)，FVB/N 品系
组织来源：卵巢表面上皮
细胞类型：上皮细胞 (Epithelial)
倍增时间：约 13小时 (其亲本 M0505 细胞约为 48 小时)
生长特性：贴壁生长

培养条件 (Culture Conditions)

基础培养基：RPMI-1640 培养基。
完全培养基配方：
- RPMI-1640 + 8%–10% 胎牛血清 (FBS)
- 1 mM 丙酮酸钠 (Sodium Pyruvate)
- 0.1 mM 非必需氨基酸 (NEAA)
- 1% 青霉素/链霉素 (可选)
培养环境： 37°C，5% CO₂，加湿培养箱。
传代方法：细胞汇合度达到 80-90% 时，使用 0.25% Trypsin-EDTA 进行消化传代。

分子与生物学特征 (Molecular Features)

癌症模型：是研究高级别浆液性卵巢癌 (HGSOC) 的重要同基因小鼠模型。
标志物表达：肿瘤组织通常呈 pan-cytokeratin (CK)+、WT1+、PAX8+，且 inhibin−。
遗传特征：伴有 Cdkn2a 缺失和 Ccnd1 (Cyclin D1) 上调，与人类 HGSOC 的基因特征高度一致。
致瘤性：在 FVB/N 小鼠腹腔注射后约 47-48 天内形成肿瘤和腹水。

常见衍生型号 (Variants)

STOSE.M1 luc：带有荧光素酶 (Luciferase) 标记的细胞系，用于 IVIS 活体成像监测肿瘤进展。

BioVector TM

The STOSE (Spontaneously Transformed Ovarian Surface Epithelial) cell line is a murine model primarily used to study high-grade serous ovarian cancer (HGSOC). It was established by spontaneously immortalising ovarian surface epithelial cells (M0505 line) from FVB/N mice through long-term propagation.

Core Specifications

Organism: Mouse (Mus musculus), FVB/N breed.
Tissue Source: Ovary, surface epithelium (in situ).
Cell Type: Epithelial.
Growth Mode: Adherent, transitioning from an epithelial "cobblestone" to a mesenchymal morphology during transformation.
Doubling Time: Approximately 13 hours (highly proliferative compared to the parental 48-hour doubling time).

Culture Conditions

While commercial data sheets may vary slightly, standard research protocols for STOSE typically use:

Basal Medium:RPMI-1640 or DMEM.
Supplements: 8–10% Fetal Bovine Serum (FBS), 1% Penicillin/Streptomycin, 1mM Sodium Pyruvate, and 0.1mM Non-Essential Amino Acids (NEAA).
Environment: 37°C in a humidified atmosphere of 5% CO₂.

Molecular & Biological Markers

HGSOC Markers: STOSE-derived tumors are typically positive for pan-cytokeratin (CK), WT1, and PAX8, while remaining negative for inhibin.
Genetic Profile: Characterised by the loss of Cdkn2a and upregulation of Ccnd1 (cyclin D1), mirroring changes in human TCGA ovarian cancer datasets.
Tumorigenicity: Forms tumors and ascites in both SCID and immunocompetent FVB/N mice within approximately 47–48 days following intraperitoneal injection.

Key Variants

STOSE.M1 luc: A luciferase-labeled version used for real-time, quantitative tracking of tumor progression and metastasis via IVIS imaging.
SCA1+ Population: A subpopulation with enhanced tumor-initiating capacity, forming palpable tumors roughly 8 days faster than SCA1- cells.