AML14.3D10 BioVector TM人造血髓系白血病嗜酸性中幼粒细胞/晚幼粒细胞株Hematopoietic-Myeloid Leukemia cell line

价　　格：¥99860
货　　号：BioVector TM -AML14.3D10
产　　地：北京

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BioVector ^TM AML14.3D10 细胞株是源自人造血髓系白血病的亚克隆，是目前研究嗜酸性粒细胞（Eosinophils）最常用的体外模型。与未分化的亲本 AML14 不同，3D10 亚系已自发分化为嗜酸性中幼粒细胞阶段。

核心参数

物种：人 (Homo sapiens)。
细胞类型：造血髓系白血病；嗜酸性中幼粒细胞/晚幼粒细胞。
形态：圆形、悬浮生长，胞质内含有丰富的嗜酸性二级颗粒。
生物安全等级： BSL-2。
倍增时间：约 24–36 小时（受密度影响较大）。

培养指南

培养基：RPMI 1640 + 8% 胎牛血清 (FBS) + 2mM L-谷氨酰胺 + 1mM 丙酮酸钠 + 50μM β-巯基乙醇。
接种密度：维持在 3 x 10⁵ 至 1 x 10⁶ cells/mL 之间。
关键点：切勿让密度超过 1 x 10⁶ cells/mL。过密会导致细胞脱颗粒，释放出的主碱性蛋白 (MBP) 等毒性物质会引发连锁死亡。
传代比例：每 3–4 天按 1:2 或 1:3 比例传代。

生物学特征

颗粒蛋白：高表达主碱性蛋白 (MBP)、嗜酸性粒细胞过氧化物酶 (EPO) 和嗜酸性粒细胞衍生神经毒素 (EDN)。
受体：表达 GM-CSF 和 IL-5 受体。需注意该亚系已失去 IL-3 受体 α 亚基。
细胞因子依赖：增殖不依赖外源细胞因子，但对 IL-5 和 GM-CSF 有明显的生物学反应。

处理与保存

冻存液： 90% FBS + 10% DMSO。
复苏注意事项：初始复苏存活率可能较低（20%–30%）。建议在复苏后的前 72 小时内增加补液频率以稀释死亡细胞释放的颗粒毒素。

BioVector TM

The AML14.3D10 cell line is a human hematopoietic myeloid leukemia-derived subclone widely used as a research model for eosinophils. Unlike the parental AML14 line, which is undifferentiated, the 3D10 subclone is a fully differentiated eosinophilic myelocyte.

Core Specifications

Organism:Homo sapiens (Human).
Cell Type: Hematopoietic-Myeloid Leukemia; specifically an eosinophilic myelocyte to metamyelocyte.
Morphology: Round, non-adherent cells containing abundant eosinophil secondary granules.
Biosafety Level: BSL-2.
Doubling Time: Approximately 24–36 hours (some sources report up to 48 hours).

Culture Conditions

Growth Medium:RPMI 1640 supplemented with 8% Fetal Bovine Serum (FBS), 2mM L-glutamine, 1mM sodium pyruvate, and 50μM (or 5 x 10⁻⁵ M) Beta-mercaptoethanol.
Seeding Density: Maintain between 3 x 10⁵ and 1 x 10⁶ cells/mL.
Critical Threshold: Do not exceed 1 x 10⁶ cells/mL. Overgrowth causes degranulation, which releases toxic proteins (like MBP) that are fatal to the culture.
Subculturing: Split every 3–4 days (typically 1 volume old culture to 2 volumes fresh media).

Key Biological Markers

Granule Proteins: Expresses high levels of major basic protein (MBP), eosinophil peroxidase (EPO), and eosinophil-derived neurotoxin (EDN).
Receptors: Expresses GM-CSF and IL-5 receptors. Note that this subclone has reportedly lost the α-subunit of the IL-3 receptor.
Growth Support: Does not require exogenous cytokines for proliferation but responds rapidly to GM-CSF and IL-5.

Handling & Storage

Cryopreservation: 90% FBS + 10% DMSO.
Thawing Caution: Initial viability is often low (20%–30%) due to granule protein toxicity. Frequent medium replacement during the first 72 hours facilitates recovery.