Azotobacter vinelandii BioVector TM维氏固氮菌-维涅兰德固氮菌-固氮能力和生产海藻酸盐(Alginate)及聚羟基脂肪酸酯(PHB)
- 价 格:¥99860
- 货 号:BioVector TM -Azotobacter vinelandii
- 产 地:北京
- BioVector NTCC典型培养物保藏中心
- 联系人:Dr.Xu, Biovector NTCC Inc.
电话:400-800-2947 工作微信:1843439339 (QQ同号)
邮件:Biovector@163.com
手机:18901268599
地址:北京
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BioVector TM Azotobacter vinelandii(维氏固氮菌)是一种大型、革兰氏阴性、好氧细菌,因其卓越的固氮能力和生产海藻酸盐(Alginate)及聚羟基脂肪酸酯(PHB)的潜力而在工业和科研中备受关注。
主要菌株技术参数DJ 菌株 (NTCC BAA-1303): 最常用的实验室标准菌株,广泛用于基因功能研究。它是 OP 菌株的衍生物,转化效率极高,由于产生固氮菌素(Azotobactin),在平板上常呈现典型的绿色。
O 菌株: 原始“野生型”菌株。其特点是菌落呈黏性或“胶状”,这源于其极高的海藻酸盐产量。
CA (或 OP) 菌株 (NTCC 13705): O 菌株的“非黏性”变体,通常用于研究固氮酶系统的生化特性。
DSM 2289 (NTCC 478):标准模式菌株,主要用于分类学鉴定和基础培养实验。
生物安全等级: BSL-1(非致病性,对人体安全)。
最佳生长温度: 26°C - 30°C。
培养基: 常用 Burk’s 培养基(无氮培养基)。若需大量生长,可添加约 2.5% 的蔗糖或甘露醇作为碳源。
形态特征: 细胞呈大卵圆形(2–4 μm),常成对出现。在逆境下可形成胞囊(Cysts),具有较强的抗干燥能力。
氧气耐受: 专性需氧菌,但具有极高的呼吸速率,以消耗细胞内的氧气从而保护其对氧敏感的固氮酶。

Azotobacter vinelandii is a large, Gram-negative, aerobic bacterium primarily known for its ability to fix atmospheric nitrogen and produce valuable biopolymers like alginate and polyhydroxyalkanoates (PHB).
Strain DJ (NTCC BAA-1303): The standard laboratory strain for genetic studies. It is highly competent for taking up foreign DNA and is a derivative of the nongummy OP strain. It typically turns agar plates green due to the production of the siderophore azotobactin.
Strain O: The "wild-type" or original strain, characterized by viscous, gummy colonies due to high alginate production.
Strain CA (or OP) (NTCC 13705): A "nongummy" version of strain O that is frequently used for studying nitrogenase systems.
DSM 2289 (NTCC 478): A type strain used for taxonomic reference and general cultivation.
Biosafety Level: BSL-1 (Non-pathogenic).
Optimum Temperature: 26°C to 30°C.
Growth Media:Modified Burk's Medium is the standard for nitrogen-free growth. On solid media, it may require sucrose or mannitol (approx. 2.5%) as a carbon source.
Morphology: Large oval cells (2–4 μm), often appearing in pairs or clusters. It can form cysts (resting cells) resistant to desiccation.
Oxygen Tolerance: While it is an obligate aerobe, it has high respiration rates to "protect" its oxygen-sensitive nitrogenase enzymes.
Supplier生产厂家:
BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
BioVector NTCC Inc.
TEL: 400-800-2947
E-mail: biovector@163.com
工作微信/QQ同号: 1843439339
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